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The epithelial calcium channel, ECaC, is activated by hyperpolarization and regulated by cytosolic calcium Hoenderop JG; van der Kemp AW; Hartog A; van Os CH; Willems PH; Bindels RJBiochem Biophys Res Commun 1999[Aug]; 261 (2): 488-92The recently cloned epithelial Ca(2+) channel, ECaC, which is expressed in the apical membrane of 1,25-dihydroxyvitamin D(3)-responsible epithelia, was characterized in Xenopus laevis oocytes by measuring the Ca(2+)-activated Cl(-) current which is a sensitive read-out of the Ca(2+) influx. ECaC-expressing oocytes responded to a voltage ramp with a maximal inward current of -2.1 +/- 0.3 microA at a holding potential of -99 +/- 1 mV. The inward current decreased progressively at less negative potentials and at +50 mV a small Ca(2+)-induced outward current was observed. The Ca(2+) influx-evoked current at a hyperpolarizing pulse to -100 mV displayed a fast activation followed by a rapid but partial inactivation. Loading of the oocytes with the Ca(2+) chelator BAPTA delayed the activation and blocked the inactivation of ECaC. When a series of brief hyperpolarizing pulses were given a significant decline in the peak response and subsequent plateau phase was observed. In conclusion, the distinct electrophysiological features of ECaC are hyperpolarization-dependent activation, Ca(2+)-dependent regulation of channel conductance and desensitization during repetitive stimulation.|Animals[MESH]|Calcium Channels/drug effects/genetics/*metabolism[MESH]|Calcium/*metabolism[MESH]|Chelating Agents/pharmacology[MESH]|Cytosol/metabolism[MESH]|Egtazic Acid/analogs & derivatives/pharmacology[MESH]|Female[MESH]|In Vitro Techniques[MESH]|Membrane Potentials[MESH]|Oocytes/drug effects/metabolism[MESH]|Rabbits[MESH]|TRPV Cation Channels[MESH]|Xenopus laevis[MESH] |
