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Evaluation of bovine embryos produced in high performance serum-free media Abe H; Hoshi HJ Reprod Dev 2003[Jun]; 49 (3): 193-202This review evaluates the quality of bovine embryos developed from in vitro-matured (IVM) and -fertilized (IVF) oocytes cultured in either serum-free or serum-containing media. Bovine embryos cultured in serum-supplemented medium contain numerous cytoplasmic lipid droplets and immature mitochondria compared to those cultured in serum-free medium. The accumulation of cytoplasmic lipids in embryos developed in serum-containing medium may be a result of incorporation of lipoproteins from the serum and may result in impaired function of mitochondria. The improved serum-free media (IVMD101 and IVD101) offer several advantages over culture in serum-containing medium, including increased rates of blastocyst formation and higher cell numbers. Additionally, the survival and hatching rates of embryos produced in serum-free media after post-thaw culture were superior to those of embryos produced in the serum-containing medium, suggesting that the abnormal accumulation of cytoplasmic lipids in embryos may have a negative effect on the sensitivity of embryos to chilling and freezing. These serum-free culture systems have proven to be beneficial for the production of good quality embryos from IVM-IVF bovine oocytes. Furthermore, recent studies have shown a correlation between mitochondrial function (oxygen consumption) and embryo quality. A new method using scanning electrochemical microscopy may be capable of assessing the viability and developmental potential of bovine embryos.|Animals[MESH]|Blastocyst/metabolism[MESH]|Cattle[MESH]|Culture Media, Serum-Free[MESH]|Embryo Culture Techniques/methods/*veterinary[MESH]|Embryo, Mammalian/cytology/*metabolism[MESH]|Female[MESH]|Fertilization in Vitro/methods/*veterinary[MESH]|Microscopy, Electron[MESH]|Morula/metabolism[MESH]|Oocytes/growth & development[MESH]|Oxygen Consumption[MESH] |
