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Technique review: how to use RNA interference Lehner B; Fraser AG; Sanderson CMBrief Funct Genomic Proteomic 2004[Apr]; 3 (1): 68-83RNA interference (RNAi) has been rapidly adopted as a general method for inhibiting gene expression in most laboratory organisms. This paper discusses how libraries of RNAi reagents are being used to perform genome-wide reverse genetic screens in both model organisms and mammalian cells. Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed.|*Genetic Techniques[MESH]|*RNA Interference[MESH]|Animals[MESH]|Cells, Cultured[MESH]|Gene Library[MESH]|Humans[MESH]|RNA, Messenger/metabolism[MESH]|RNA, Small Interfering/metabolism[MESH]|Recombination, Genetic[MESH] |