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Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport Lambers TT; Mahieu F; Oancea E; Hoofd L; de Lange F; Mensenkamp AR; Voets T; Nilius B; Clapham DE; Hoenderop JG; Bindels RJEMBO J 2006[Jul]; 25 (13): 2978-88In Ca(2+)-transporting epithelia, calbindin-D(28K) (CaBP(28K)) facilitates Ca(2+) diffusion from the luminal Ca(2+) entry side of the cell to the basolateral side, where Ca(2+) is extruded into the extracellular compartment. Simultaneously, CaBP(28K) provides protection against toxic high Ca(2+) levels by buffering the cytosolic Ca(2+) concentration ([Ca(2+)](i)) during high Ca(2+) influx. CaBP(28K) consistently colocalizes with the epithelial Ca(2+) channel TRPV5, which constitutes the apical entry step in renal Ca(2+)-transporting epithelial cells. Here, we demonstrate using protein-binding analysis, subcellular fractionation and evanescent-field microscopy that CaBP(28K) translocates towards the plasma membrane and directly associates with TRPV5 at a low [Ca(2+)](i). (45)Ca(2+) uptake measurements, electrophysiological recordings and transcellular Ca(2+) transport assays of lentivirus-infected primary rabbit connecting tubule/distal convolute tubule cells revealed that associated CaBP(28K) tightly buffers the flux of Ca(2+) entering the cell via TRPV5, facilitating high Ca(2+) transport rates by preventing channel inactivation. In summary, CaBP(28K) acts in Ca(2+)-transporting epithelia as a dynamic Ca(2+) buffer, regulating [Ca(2+)] in close vicinity to the TRPV5 pore by direct association with the channel.|Animals[MESH]|Calbindin 1[MESH]|Calbindins[MESH]|Calcium/*physiology[MESH]|Cell Membrane/*metabolism[MESH]|Cells, Cultured[MESH]|Dogs[MESH]|Epithelial Cells/metabolism[MESH]|Humans[MESH]|Ion Transport[MESH]|Kidney Tubules/cytology/metabolism[MESH]|Protein Binding[MESH]|Protein Transport[MESH]|Rabbits[MESH]|Recombinant Proteins/metabolism[MESH]|S100 Calcium Binding Protein G/*physiology[MESH]|TRPV Cation Channels/*physiology[MESH]|Urothelium/cytology/metabolism[MESH] |
