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Experimental approaches for solution X-ray scattering and fiber diffraction Tsuruta H; Irving TCCurr Opin Struct Biol 2008[Oct]; 18 (5): 601-8X-ray scattering and diffraction from non-crystalline systems have gained renewed interest in recent years, as focus shifts from the structural chemistry information gained by high-resolution studies to the context of structural physiology at larger length scales. Such techniques permit the study of isolated macromolecules as well as highly organized macromolecular assemblies as a whole under near-physiological conditions. Time-resolved approaches, made possible by advanced synchrotron instrumentation, add a crucial dimension to many of these investigations. This article reviews experimental approaches in non-crystalline X-ray scattering and diffraction that may be used to illuminate important scientific questions such as protein/nucleic acid folding and structure-function relationships in large macromolecular assemblies.|Animals[MESH]|Connective Tissue/ultrastructure[MESH]|Crystallography, X-Ray/instrumentation/methods[MESH]|Drosophila[MESH]|Models, Molecular[MESH]|Molecular Conformation[MESH]|Neutrons[MESH]|Nucleic Acids/*chemistry[MESH]|Pharmaceutical Solutions[MESH]|Protein Conformation[MESH]|Proteins/*chemistry[MESH]|Scattering, Radiation[MESH]|Solutions[MESH]|Synchrotrons[MESH]|X-Ray Diffraction/*instrumentation/methods[MESH] |
