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Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow Leuba SH; Wheeler TB; Cheng CM; LeDuc PR; Fernandez-Sierra M; Quinones EMethods 2009[Mar]; 47 (3): 214-22Here we describe the use of magnetic tweezers and or microfluidics to manipulate single DNA molecules. We describe experiment which employ magnetic tweezers coupled to an inverted microscope as well as the use of a magnetic tweezers setup with an upright microscope. Using a chamber prepared via soft lithography, we also describe a microfluidic device for the manipulation of individual DNA molecules. Finally, we present some past successful examples of using these approaches to elucidate unique information about protein-nucleic acid interactions.|*Nucleic Acid Conformation[MESH]|Chromatin Assembly and Disassembly[MESH]|DNA/*chemistry/metabolism[MESH]|Exodeoxyribonucleases/chemistry[MESH]|Magnetics/*instrumentation/methods[MESH]|Microfluidic Analytical Techniques/instrumentation/*methods[MESH]|Microfluidics[MESH]|Microscopy, Video/instrumentation/methods[MESH]|Transcription, Genetic[MESH] |
