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Identification and analysis of new proteins involved in the DNA damage response network of Fanconi anemia and Bloom syndrome Guo R; Xu D; Wang WMethods 2009[May]; 48 (1): 72-9The use of co-immunoprecipitation (co-IP) to purify multi-protein complexes has contributed greatly to our understanding of the DNA damage response network associated with Fanconi anemia (FA), Bloom syndrome (BS) and breast cancer. Four new FA genes and two new protein partners for the Bloom syndrome gene product have been identified by co-IP. Here, we discuss our experience in using co-IP and other techniques to isolate and characterize new FA and BS-related proteins.|*DNA Damage[MESH]|Bloom Syndrome/*genetics/metabolism[MESH]|Carrier Proteins/*analysis/isolation & purification/metabolism[MESH]|DNA Topoisomerases, Type I/*analysis/isolation & purification/metabolism[MESH]|DNA-Binding Proteins/*analysis/isolation & purification/metabolism[MESH]|Fanconi Anemia/*genetics/metabolism[MESH]|Humans[MESH]|Nuclear Proteins/*analysis/isolation & purification/metabolism[MESH]|RecQ Helicases/*analysis/isolation & purification/metabolism[MESH] |
