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lüll Morphogen gradient formation Wartlick O; Kicheva A; Gonzalez-Gaitan MCold Spring Harb Perspect Biol 2009[Sep]; 1 (3): a001255How morphogen gradients are formed in target tissues is a key question for understanding the mechanisms of morphological patterning. Here, we review different mechanisms of morphogen gradient formation from theoretical and experimental points of view. First, a simple, comprehensive overview of the underlying biophysical principles of several mechanisms of gradient formation is provided. We then discuss the advantages and limitations of different experimental approaches to gradient formation analysis.|Animals[MESH]|Body Patterning/*physiology[MESH]|Cell Biology[MESH]|Cell Differentiation[MESH]|Cell Lineage[MESH]|Developmental Biology[MESH]|Diffusion[MESH]|Green Fluorescent Proteins/metabolism[MESH]|Humans[MESH]|Kinetics[MESH]|Models, Biological[MESH]|Models, Statistical[MESH]|Morphogenesis/*genetics[MESH]|Mutation[MESH] |