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DNA diagnostics: nanotechnology-enhanced electrochemical detection of nucleic acids Wei F; Lillehoj PB; Ho CMPediatr Res 2010[May]; 67 (5): 458-68The detection of mismatched base pairs in DNA plays a crucial role in the diagnosis of genetic-related diseases and conditions, especially for early stage treatment. Among the various biosensors that have been used for DNA detection, EC sensors show great promise because they are capable of precise DNA recognition and efficient signal transduction. Advancements in micro- and nanotechnologies, specifically fabrication techniques and new nanomaterials, have enabled for the development of highly sensitive, highly specific sensors making them attractive for the detection of small sequence variations. Furthermore, the integration of sensors with sample preparation and fluidic processes enables for rapid, multiplexed DNA detection essential for POC clinical diagnostics.|*Electrochemical Techniques/instrumentation[MESH]|*Genetic Testing[MESH]|*Nanotechnology/instrumentation[MESH]|Biosensing Techniques[MESH]|DNA Mutational Analysis[MESH]|DNA/*analysis[MESH]|Equipment Design[MESH]|Genetic Predisposition to Disease[MESH]|Humans[MESH]|Nanomedicine/instrumentation/*methods[MESH]|Point-of-Care Systems[MESH]|Predictive Value of Tests[MESH]|Sensitivity and Specificity[MESH] |
