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Imaging mobile zinc in biology Tomat E; Lippard SJCurr Opin Chem Biol 2010[Apr]; 14 (2): 225-30Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.|Animals[MESH]|Biosensing Techniques/*methods[MESH]|Fluorescent Dyes/analysis/chemistry[MESH]|Humans[MESH]|Microscopy, Fluorescence/methods[MESH]|Spectrometry, Fluorescence/methods[MESH]|Zinc/*analysis/*metabolism[MESH] |
