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Studying bacterial transcriptomes using RNA-seq Croucher NJ; Thomson NRCurr Opin Microbiol 2010[Oct]; 13 (5): 619-24Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms. RNA-seq has a number of advantages over hybridization-based techniques, such as annotation-independent detection of transcription, improved sensitivity and increased dynamic range. Early studies have uncovered a wealth of novel coding sequences and non-coding RNA, and are revealing a transcriptional landscape that increasingly mirrors that of eukaryotes. Already basic RNA-seq protocols have been improved and adapted to looking at particular aspects of RNA biology, often with an emphasis on non-coding RNAs, and further refinements to current techniques will improve our understanding of gene expression, and genome content, in the future.|*Gene Expression Profiling[MESH]|Genes, Bacterial[MESH]|Oligonucleotide Array Sequence Analysis[MESH]|RNA, Bacterial/*analysis/genetics[MESH]|Sequence Analysis, RNA/*methods[MESH] |