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10.3390/microarrays4010064 http://scihub22266oqcxt.onion/10.3390/microarrays4010064 C4996383!4996383!27600213     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27600213.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Microarrays+(Basel) 2015 ; 4 (1): 64-83 Nephropedia Template TP {{tp|p=27600213|t=2015. 3D Cultivation Techniques for Primary Human Hepatocytes |pdf=|usr=}}{{27600213}} gab.com Text 3D Cultivation Techniques for Primary Human Hepatocytes JO: Microarrays (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=27600213 #FOAvip One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions result in a loss of hepatocyte morphology and of liver-specific functions, such as drug-metabolizing enzymes and transporters. As it has been demonstrated that hepatocytes embedded between two sheets of collagen maintain their function, various hydrogels and scaffolds for the 3D cultivation of hepatocytes have been developed. To further improve or maintain hepatic functions, 3D cultivation has been combined with perfusion. In this manuscript, we discuss the benefits and drawbacks of different 3D microfluidic devices. For most systems that are currently available, the main issues are the requirement of large cell numbers, the low throughput, and expensive equipment, which render these devices unattractive for research and the drug-developing industry. A higher acceptance of these devices could be achieved by their simplification and their compatibility with high-throughput, as both aspects are of major importance for a user-friendly device. Twit Text FOAVip 3D Cultivation Techniques for Primary Human Hepatocytes ????Microarrays (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=27600213 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27600213 #FOAvip English Wikipedia <ref>{{Cite pmid|27600213}}</ref> 3D Cultivation Techniques for Primary Human Hepatocytes #MMPMID27600213 Bachmann A; Moll M; Gottwald E; Nies C; Zantl R; Wagner H; Burkhardt B; Sánchez JJM; Ladurner R; Thasler W; Damm G; Nussler AK Microarrays (Basel) 2015[Mar]; 4 (1): 64-83 PMID27600213show ga One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions result in a loss of hepatocyte morphology and of liver-specific functions, such as drug-metabolizing enzymes and transporters. As it has been demonstrated that hepatocytes embedded between two sheets of collagen maintain their function, various hydrogels and scaffolds for the 3D cultivation of hepatocytes have been developed. To further improve or maintain hepatic functions, 3D cultivation has been combined with perfusion. In this manuscript, we discuss the benefits and drawbacks of different 3D microfluidic devices. For most systems that are currently available, the main issues are the requirement of large cell numbers, the low throughput, and expensive equipment, which render these devices unattractive for research and the drug-developing industry. A higher acceptance of these devices could be achieved by their simplification and their compatibility with high-throughput, as both aspects are of major importance for a user-friendly device. ä3D Cultivation Techniques for Primary Human Hepatocytes (epmc).pdf DeepDyve Pubget Overpricing