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10.1080/16512235.2017.1345574 http://scihub22266oqcxt.onion/10.1080/16512235.2017.1345574 C5614383!5614383!28959177     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28959177&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28959177.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Microb+Ecol+Health+Dis 2017 ; 28 (1): ä Nephropedia Template TP {{tp|p=28959177|t=2017. Ancient permafrost staphylococci carry antibiotic resistance genes |pdf=|usr=}}{{28959177}} gab.com Text Ancient permafrost staphylococci carry antibiotic resistance genes JO: Microb Ecol Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=28959177 #FOAvip Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years.Objective: To cultivate bacterial strains from permafrost.Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40?60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences.Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome.Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance. Twit Text FOAVip Ancient permafrost staphylococci carry antibiotic resistance genes ????Microb Ecol Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=28959177 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28959177 #FOAvip English Wikipedia <ref>{{Cite pmid|28959177}}</ref> Ancient permafrost staphylococci carry antibiotic resistance genes #MMPMID28959177 Kashuba E; Dmitriev AA; Kamal SM; Melefors O; Griva G; Römling U; Ernberg I; Kashuba V; Brouchkov A Microb Ecol Health Dis 2017[]; 28 (1): ä PMID28959177show ga Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years.Objective: To cultivate bacterial strains from permafrost.Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40?60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences.Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome.Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance. äAncient permafrost staphylococci carry antibiotic resistance genes (epmc).pdf DeepDyve Pubget Overpricing