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10.1371/journal.pone.0195010 http://scihub22266oqcxt.onion/10.1371/journal.pone.0195010 C5875863!5875863!29596514     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29596514&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29596514.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2018 ; 13 (3): ä Nephropedia Template TP {{tp|p=29596514|t=2018. Characterisation of insulin analogues therapeutically available to patients |pdf=|usr=}}{{29596514}} gab.com Text Characterisation of insulin analogues therapeutically available to patients JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29596514 #FOAvip The structure and function of clinical dosage insulin and its analogues were assessed. This included ?native insulins? (human recombinant, bovine, porcine), ?fast-acting analogues? (aspart, glulisine, lispro) and ?slow-acting analogues? (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity and equilibrium experiments, were employed to yield distributions of both molar mass and sedimentation coefficient of all nine insulins. Size exclusion chromatography, coupled to multi-angle light scattering, was also used to explore the function of these analogues. On ultracentrifugation analysis, the insulins under investigation were found to be in numerous conformational states, however the majority of insulins were present in a primarily hexameric conformation. This was true for all native insulins and two fast-acting analogues. However, glargine was present as a dimer, detemir was a multi-hexameric system, degludec was a dodecamer (di-hexamer) and glulisine was present as a dimer-hexamer-dihexamer system. However, size-exclusion chromatography showed that the two hexameric fast-acting analogues (aspart and lispro) dissociated into monomers and dimers due to the lack of zinc in the mobile phase. This comprehensive study is the first time all nine insulins have been characterised in this way, the first time that insulin detemir have been studied using analytical ultracentrifugation and the first time that insulins aspart and glulisine have been studied using sedimentation equilibrium. The structure and function of these clinically administered insulins is of critical importance and this research adds novel data to an otherwise complex functional physiological protein. Twit Text FOAVip Characterisation of insulin analogues therapeutically available to patients ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29596514 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29596514 #FOAvip English Wikipedia <ref>{{Cite pmid|29596514}}</ref> Characterisation of insulin analogues therapeutically available to patients #MMPMID29596514 Adams GG; Meal A; Morgan PS; Alzahrani QE; Zobel H; Lithgo R; Kok MS; Besong DTM; Jiwani SI; Ballance S; Harding SE; Chayen N; Gillis RB PLoS One 2018[]; 13 (3): ä PMID29596514show ga The structure and function of clinical dosage insulin and its analogues were assessed. This included ?native insulins? (human recombinant, bovine, porcine), ?fast-acting analogues? (aspart, glulisine, lispro) and ?slow-acting analogues? (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity and equilibrium experiments, were employed to yield distributions of both molar mass and sedimentation coefficient of all nine insulins. Size exclusion chromatography, coupled to multi-angle light scattering, was also used to explore the function of these analogues. On ultracentrifugation analysis, the insulins under investigation were found to be in numerous conformational states, however the majority of insulins were present in a primarily hexameric conformation. This was true for all native insulins and two fast-acting analogues. However, glargine was present as a dimer, detemir was a multi-hexameric system, degludec was a dodecamer (di-hexamer) and glulisine was present as a dimer-hexamer-dihexamer system. However, size-exclusion chromatography showed that the two hexameric fast-acting analogues (aspart and lispro) dissociated into monomers and dimers due to the lack of zinc in the mobile phase. This comprehensive study is the first time all nine insulins have been characterised in this way, the first time that insulin detemir have been studied using analytical ultracentrifugation and the first time that insulins aspart and glulisine have been studied using sedimentation equilibrium. The structure and function of these clinically administered insulins is of critical importance and this research adds novel data to an otherwise complex functional physiological protein. äCharacterisation of insulin analogues therapeutically available to pat(epmc).pdf DeepDyve Pubget Overpricing