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10.1016/j.carbpol.2014.10.054 http://scihub22266oqcxt.onion/10.1016/j.carbpol.2014.10.054 C4379424!4379424!25817684     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25817684.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Carbohydr+Polym 2015 ; 122 (ä): 399-407 Nephropedia Template TP {{tp|p=25817684|t=2015. Combinatorial one-pot chemoenzymatic synthesis of heparin |pdf=|usr=}}{{25817684}} gab.com Text Combinatorial one-pot chemoenzymatic synthesis of heparin JO: Carbohydr Polym http://www.kidney.de/pget.php?&tw=1&pmid=25817684 #FOAvip Contamination in heparin batches during early 2008 has resulted in a significant effort to develop a safer bioengineered heparin using bacterial capsular polysaccharide heparosan and recombinant enzymes derived from the heparin/heparan sulfate biosynthetic pathway. This requires controlled chemical N-deacetylation/ N-sulfonation of heparosan followed by epimerization of most of its glucuronic acid residues to iduronic acid and O-sulfation of the C2 position of iduronic acid and the C3 and C6 positions of the glucosamine residues. A combinatorial study of multi-enzyme, one-pot, in vitro biocatalytic synthesis, carried out in tandem with sensitive analytical techniques, reveals controlled structural changes leading to heparin products similar to animal- derived heparin active pharmaceutical ingredients. Liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analysis confirms an abundance of heparin?s characteristic trisulfated disaccharide, as well as 3-O-sulfo containing residues critical for heparin binding to antithrombin III and its anticoagulant activity. The bioengineered heparins prepared using this simplified one-pot chemoenzymatic synthesis also show in vitro anticoagulant activity. Twit Text FOAVip Combinatorial one-pot chemoenzymatic synthesis of heparin ????Carbohydr Polym http://www.kidney.de/pget.php?&tw=1&pmid=25817684 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25817684 #FOAvip English Wikipedia <ref>{{Cite pmid|25817684}}</ref> Combinatorial one-pot chemoenzymatic synthesis of heparin #MMPMID25817684 Bhaskar U; Li G; Fu L; Onishi A; Suflita M; Dordick JS; Linhardt RJ Carbohydr Polym 2015[May]; 122 (ä): 399-407 PMID25817684show ga Contamination in heparin batches during early 2008 has resulted in a significant effort to develop a safer bioengineered heparin using bacterial capsular polysaccharide heparosan and recombinant enzymes derived from the heparin/heparan sulfate biosynthetic pathway. This requires controlled chemical N-deacetylation/ N-sulfonation of heparosan followed by epimerization of most of its glucuronic acid residues to iduronic acid and O-sulfation of the C2 position of iduronic acid and the C3 and C6 positions of the glucosamine residues. A combinatorial study of multi-enzyme, one-pot, in vitro biocatalytic synthesis, carried out in tandem with sensitive analytical techniques, reveals controlled structural changes leading to heparin products similar to animal- derived heparin active pharmaceutical ingredients. Liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analysis confirms an abundance of heparin?s characteristic trisulfated disaccharide, as well as 3-O-sulfo containing residues critical for heparin binding to antithrombin III and its anticoagulant activity. The bioengineered heparins prepared using this simplified one-pot chemoenzymatic synthesis also show in vitro anticoagulant activity. äCombinatorial one-pot chemoenzymatic synthesis of heparin (epmc).pdf DeepDyve Pubget Overpricing