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Development of the Resolution Theory for Electrophoretic Exclusion #MMPMID24916305
Kenyon SM; Keebaugh MW; Hayes MA
Electrophoresis 2014[Sep]; 35 (18): 2551-9 PMID24916305show ga
Electrophoretic exclusion, a technique that differentiates species in bulk solution near a channel entrance, has been demonstrated on benchtop and microdevice designs. In these systems, separation occurs when the electrophoretic velocity of one species is greater than the opposing hydrodynamic flow, while the velocity of the other species is less than that flow. Although exclusion has been demonstrated in multiple systems for a range of analytes, a theoretical assessment of resolution has not been addressed. To compare the results of these calculations to traditional techniques, the performance is expressed in terms of smallest difference in electrophoretic mobilities that can be completely separated (R = 1.5). The calculations indicate that closest resolvable species (??min) differ by approximately 10?13 m2/Vs and peak capacity (nc) is 1000. Published experimental data is compared to these calculated results.