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10.1038/ncomms8513 http://scihub22266oqcxt.onion/10.1038/ncomms8513 C4491823!4491823!26104298     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26104298&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26104298.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2015 ; 6 (ä): ä Nephropedia Template TP {{tp|p=26104298|t=2015. Digital microfluidic immunocytochemistry in single cells |pdf=|usr=}}{{26104298}} gab.com Text Digital microfluidic immunocytochemistry in single cells JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26104298 #FOAvip We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus for as little as 3?s. DISC was used to probe the phosphorylation states of platelet-derived growth factor receptor (PDGFR) and the downstream signalling protein, Akt, to evaluate concentration- and time-dependent effects of stimulation. The high time resolution of the technique allowed for surprising new observations?for example, a 10?s pulse stimulus of a low concentration of PDGF is sufficient to cause >30% of adherent fibroblasts to commit to Akt activation. With the ability to quantitatively probe signalling events with high time resolution at the single-cell level, we propose that DISC may be an important new technique for a wide range of applications, especially for screening signalling responses of a heterogeneous cell population. Twit Text FOAVip Digital microfluidic immunocytochemistry in single cells ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26104298 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26104298 #FOAvip English Wikipedia <ref>{{Cite pmid|26104298}}</ref> Digital microfluidic immunocytochemistry in single cells #MMPMID26104298 Ng AHC; Chamberlain MD; Situ H; Lee V; Wheeler AR Nat Commun 2015[]; 6 (ä): ä PMID26104298show ga We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus for as little as 3?s. DISC was used to probe the phosphorylation states of platelet-derived growth factor receptor (PDGFR) and the downstream signalling protein, Akt, to evaluate concentration- and time-dependent effects of stimulation. The high time resolution of the technique allowed for surprising new observations?for example, a 10?s pulse stimulus of a low concentration of PDGF is sufficient to cause >30% of adherent fibroblasts to commit to Akt activation. With the ability to quantitatively probe signalling events with high time resolution at the single-cell level, we propose that DISC may be an important new technique for a wide range of applications, especially for screening signalling responses of a heterogeneous cell population. äDigital microfluidic immunocytochemistry in single cells (epmc).pdf DeepDyve Pubget Overpricing