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10.1364/BOE.7.000399

http://scihub22266oqcxt.onion/10.1364/BOE.7.000399
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C4771458!4771458!26977349
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suck abstract from ncbi


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pmid26977349      Biomed+Opt+Express 2016 ; 7 (2): 399-408
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  • Fast interferometric second harmonic generation microscopy #MMPMID26977349
  • Bancelin S; Couture CA; Légaré K; Pinsard M; Rivard M; Brown C; Légaré F
  • Biomed Opt Express 2016[Feb]; 7 (2): 399-408 PMID26977349show ga
  • We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.
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