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10.2174/1566523216666160331130040 http://scihub22266oqcxt.onion/10.2174/1566523216666160331130040 C5412002!5412002!27029943     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27029943&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27029943.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Curr+Gene+Ther 2016 ; 16 (2): 98-129 Nephropedia Template TP {{tp|p=27029943|t=2016. Gene Electrotransfer: A Mechanistic Perspective |pdf=|usr=}}{{27029943}} gab.com Text Gene Electrotransfer: A Mechanistic Perspective JO: Curr Gene Ther http://www.kidney.de/pget.php?&tw=1&pmid=27029943 #FOAvip Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer. Twit Text FOAVip Gene Electrotransfer: A Mechanistic Perspective ????Curr Gene Ther http://www.kidney.de/pget.php?&tw=1&pmid=27029943 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27029943 #FOAvip English Wikipedia <ref>{{Cite pmid|27029943}}</ref> Gene Electrotransfer: A Mechanistic Perspective #MMPMID27029943 Rosazza C; Meglic SH; Zumbusch A; Rols MP; Miklavcic D Curr Gene Ther 2016[Apr]; 16 (2): 98-129 PMID27029943show ga Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer. äGene Electrotransfer: A Mechanistic Perspective (epmc).pdf DeepDyve Pubget Overpricing