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http://scihub22266oqcxt.onion/ C5952195!5952195 !1726467     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\1726467 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Gene+Expr 1991 ; 1 (1 ): 5-14 Nephropedia Template TP {{tp|p=1726467 |t=1991. Polyadenylation precedes splicing in vitro |pdf=|usr=}}{{1726467 }} gab.com Text Polyadenylation precedes splicing in vitro JO: Gene Expr http://www.kidney.de/pget.php?&tw=1&pmid=1726467 #FOAvip Vertebrate premessenger RNAs are usually spliced and polyadenylated. In vivo analysis of the relative kinetics of the two reactions is difficult. We have used in vitro processing systems to investigate the order of splicing and polyadenylation of chimeric precursor RNAs containing a single intron and a poly(A) site. Polyadenylated, but not spliced, intermediate RNA appeared first and reached a low steady-state level early during incubation, properties consistent with its being a reaction intermediate in the production of doubly-processed spliced and polyadenylated product RNA. The kinetics of polyadenylation suggested that polyadenylated RNA was the only intermediate in the production of doubly-processed RNA. Spliced, but not polyadenylated, RNA also appeared. This species, however, continued to accumulate during reaction, and could not be chased into product spliced and polyadenylated RNA. These data support a preferred order of reaction for 3' terminal introns and exons in which polyadenylation precedes splicing. Twit Text FOAVip Polyadenylation precedes splicing in vitro ????Gene Expr http://www.kidney.de/pget.php?&tw=1&pmid=1726467 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=1726467 #FOAvip English Wikipedia <ref>{{Cite pmid|1726467 }}</ref> Polyadenylation precedes splicing in vitro #MMPMID1726467 Niwa M ; Berget SM Gene Expr 1991[Apr]; 1 (1 ): 5-14 PMID1726467 show ga Vertebrate premessenger RNAs are usually spliced and polyadenylated. In vivo analysis of the relative kinetics of the two reactions is difficult. We have used in vitro processing systems to investigate the order of splicing and polyadenylation of chimeric precursor RNAs containing a single intron and a poly(A) site. Polyadenylated, but not spliced, intermediate RNA appeared first and reached a low steady-state level early during incubation, properties consistent with its being a reaction intermediate in the production of doubly-processed spliced and polyadenylated product RNA. The kinetics of polyadenylation suggested that polyadenylated RNA was the only intermediate in the production of doubly-processed RNA. Spliced, but not polyadenylated, RNA also appeared. This species, however, continued to accumulate during reaction, and could not be chased into product spliced and polyadenylated RNA. These data support a preferred order of reaction for 3' terminal introns and exons in which polyadenylation precedes splicing. |Adenoviridae [MESH] |Exons [MESH] |Genes, Synthetic [MESH] |Kinetics [MESH] |Magnesium [MESH] |Poly A/*metabolism [MESH] |RNA Precursors/*metabolism [MESH] |RNA Splicing/*physiology [MESH] |RNA, Messenger [MESH] |RNA/metabolism [MESH] |Regulatory Sequences, Nucleic Acid [MESH] |Ribonucleoproteins/metabolism [MESH] |Simian virus 40 [MESH]Polyadenylation precedes splicing in vitro (epmc).pdf DeepDyve Pubget Overpricing