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10.1100/tsw.2002.846

http://scihub22266oqcxt.onion/10.1100/tsw.2002.846
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C6009391!6009391!12806159
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suck abstract from ncbi


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pmid12806159      ScientificWorldJournal 2002 ; 2 (�): 1662-6
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  • Purification of Lipid Rafts from Cultured Cells #MMPMID12806159
  • Graham J
  • ScientificWorldJournal 2002[]; 2 (�): 1662-6 PMID12806159show ga
  • Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutions contain 0.5?1.0% Triton X-100, the intact lipid rafts float to the top of the gradient while all of the other detergent-solubilized membranes remain at the bottom.



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