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10.1155/2018/9576959 http://scihub22266oqcxt.onion/10.1155/2018/9576959 C6022269!6022269!30013601     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30013601&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\30013601.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Stem+Cells+Int 2018 ; 2018 (ä): ä Nephropedia Template TP {{tp|p=30013601|t=2018. User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9 |pdf=|usr=}}{{30013601}} gab.com Text User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9 JO: Stem Cells Int http://www.kidney.de/pget.php?&tw=1&pmid=30013601 #FOAvip Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously. Collectively, a simple and rapid method was presented to knock out any DNA element conditionally. This approach will facilitate the functional studies of essential genes and regulatory regions during development. Twit Text FOAVip User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9 ????Stem Cells Int http://www.kidney.de/pget.php?&tw=1&pmid=30013601 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30013601 #FOAvip English Wikipedia <ref>{{Cite pmid|30013601}}</ref> User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9 #MMPMID30013601 Chen L; Ye Y; Dai H; Zhang H; Zhang X; Wu Q; Zhu Z; Spalinskas R; Ren W; Zhang W Stem Cells Int 2018[]; 2018 (ä): ä PMID30013601show ga Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously. Collectively, a simple and rapid method was presented to knock out any DNA element conditionally. This approach will facilitate the functional studies of essential genes and regulatory regions during development. äUser-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing