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10.1006/viro.2002.1585 http://scihub22266oqcxt.onion/10.1006/viro.2002.1585 12359439!ä!12359439 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\12359439.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Virology 2002 ; 301 (2): 374-80 Nephropedia Template TP {{tp|p=12359439|t=2002. Identification and characterization of a second novel human erythrovirus variant, A6 |pdf=|usr=}}{{12359439}} gab.com Text Identification and characterization of a second novel human erythrovirus variant, A6 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=12359439 #FOAvip Parvovirus B19 (B19), currently the only accepted member of the Erythrovirus genus, is the only parvovirus known to be pathogenic in humans. Recently a viral sequence, tentatively termed V9 which showed 11% variability from the published B19 sequences, was described from a patient with aplastic crisis. To search for additional parvovirus variants, we used the new NS1/7.5EC PCR assay whose primers were designed from a conserved region of the B19/V9 sequence and encompasses an MfeI restriction enzyme site that would allow differentiation between B19- and V9-like sequences. Screening of 225 serum and bone marrow samples and 62 plasma pools identified one new atypical parvovirus sequence, A6, from an anemic HIV-positive patient. A6 exhibited 88% similarity to B19 and 92% to V9, compared to >98% correspondence between reported B19 isolates. Based on the genome similarity to B19, an RT-PCR for A6 capsid transcripts was developed and used to test for A6 infectivity of UT7/Epo/S1 cells. Despite high viral titers, A6 viral transcripts were not detected. Thus, although the prevalence of B19 variants probably is low, the true clinical significance remains unknown. Current PCR analyses are unlikely to detect novel variants without the design of specific primers to the A6/V9/B19 common sequences. Twit Text FOAVip Identification and characterization of a second novel human erythrovirus variant, A6 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=12359439 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12359439 #FOAvip English Wikipedia <ref>{{Cite pmid|12359439}}</ref> Identification and characterization of a second novel human erythrovirus variant, A6 #MMPMID12359439 Nguyen QT; Wong S; Heegaard ED; Brown KE Virology 2002[Sep]; 301 (2): 374-80 PMID12359439show ga Parvovirus B19 (B19), currently the only accepted member of the Erythrovirus genus, is the only parvovirus known to be pathogenic in humans. Recently a viral sequence, tentatively termed V9 which showed 11% variability from the published B19 sequences, was described from a patient with aplastic crisis. To search for additional parvovirus variants, we used the new NS1/7.5EC PCR assay whose primers were designed from a conserved region of the B19/V9 sequence and encompasses an MfeI restriction enzyme site that would allow differentiation between B19- and V9-like sequences. Screening of 225 serum and bone marrow samples and 62 plasma pools identified one new atypical parvovirus sequence, A6, from an anemic HIV-positive patient. A6 exhibited 88% similarity to B19 and 92% to V9, compared to >98% correspondence between reported B19 isolates. Based on the genome similarity to B19, an RT-PCR for A6 capsid transcripts was developed and used to test for A6 infectivity of UT7/Epo/S1 cells. Despite high viral titers, A6 viral transcripts were not detected. Thus, although the prevalence of B19 variants probably is low, the true clinical significance remains unknown. Current PCR analyses are unlikely to detect novel variants without the design of specific primers to the A6/V9/B19 common sequences. |*Genetic Variation[MESH] |Base Sequence[MESH] |Cell Line[MESH] |Cloning, Molecular[MESH] |DNA, Viral[MESH] |Erythrovirus/*genetics/physiology[MESH] |Humans[MESH] |Molecular Sequence Data[MESH] |Nucleic Acid Hybridization/methods[MESH] |Parvoviridae Infections/virology[MESH] |Parvovirus B19, Human/genetics/physiology[MESH] |Plasmids[MESH] |Polymerase Chain Reaction/methods[MESH] |Sequence Homology, Amino Acid[MESH]Identification and characterization of a second novel human erythrovir(epmc).pdf DeepDyve Pubget Overpricing