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10.1128/JVI.80.3.1604-1609.2006

http://scihub22266oqcxt.onion/10.1128/JVI.80.3.1604-1609.2006
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suck abstract from ncbi


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pmid16415037      J+Virol 2006 ; 80 (3): 1604-9
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  • Identification and characterization of two internal cleavage and polyadenylation sites of parvovirus B19 RNA #MMPMID16415037
  • Yoto Y; Qiu J; Pintel DJ
  • J Virol 2006[Feb]; 80 (3): 1604-9 PMID16415037show ga
  • Polyadenylation of B19 pre-mRNAs at the major internal site, (pA)p1, is programmed by the nonconsensus core cleavage and polyadenylation specificity factor-binding hexanucleotide AUUAAA. Efficient use of this element requires both downstream and upstream cis-acting elements and is further influenced by an adjacent AAUAAC motif. The primary hexanucleotide element must be nonconsensus to allow efficient readthrough of P6-generated pre-mRNAs into the capsid-coding region. An additional cleavage and polyadenylation site, (pA)p2, 296 nucleotides downstream of (pA)p1 was shown to be used following both B19 infection and transfection of a genomic clone. RNAs polyadenylated at (pA)p2 comprise approximately 10% of B19 RNAs that are polyadenylated internally.
  • |Alternative Splicing[MESH]
  • |Animals[MESH]
  • |Base Sequence[MESH]
  • |Binding Sites/genetics[MESH]
  • |COS Cells[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Humans[MESH]
  • |Molecular Sequence Data[MESH]
  • |Parvovirus B19, Human/*genetics/*metabolism[MESH]
  • |RNA Precursors/*genetics/*metabolism[MESH]
  • |RNA, Viral/*genetics/*metabolism[MESH]


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