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10.1128/JCM.01904-07 http://scihub22266oqcxt.onion/10.1128/JCM.01904-07 18160451!2268373!18160451     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=18160451&cmd=llinks): Failed to open stream: HTTP request failed! 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Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus |pdf=|usr=}}{{18160451}} gab.com Text Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=18160451 #FOAvip The common respiratory viruses, including influenza A, influenza B, and newly emerging severe acute respiratory syndrome (SARS) viruses, may cause similar clinical symptoms. Therefore, differential diagnosis of these virus pathogens is frequently required for single clinical samples. In addition, there is an urgent need for noninfectious and stable RNA standards and controls for multivirus detection. In this study, reverse transcription-PCR (RT-PCR) targeting of the RNAs of influenza A and influenza B viruses and SARS coronavirus was performed, and the resulting products were spliced into a fragment which was packaged into armored RNA for use as a noninfectious, quantifiable synthetic substitute. Furthermore, in the present study we developed a multiplex real-time RT-PCR assay in which the armored RNA was used as an external positive control and the three RNA viruses could be detected simultaneously in a single reaction mix. The detection limit of the multiplex real-time PCR was 10 copies/microl of armored RNA. Twit Text FOAVip Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=18160451 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18160451 #FOAvip English Wikipedia <ref>{{Cite pmid|18160451}}</ref> Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus #MMPMID18160451 Yu XF; Pan JC; Ye R; Xiang HQ; Kou Y; Huang ZC J Clin Microbiol 2008[Mar]; 46 (3): 837-41 PMID18160451show ga The common respiratory viruses, including influenza A, influenza B, and newly emerging severe acute respiratory syndrome (SARS) viruses, may cause similar clinical symptoms. Therefore, differential diagnosis of these virus pathogens is frequently required for single clinical samples. In addition, there is an urgent need for noninfectious and stable RNA standards and controls for multivirus detection. In this study, reverse transcription-PCR (RT-PCR) targeting of the RNAs of influenza A and influenza B viruses and SARS coronavirus was performed, and the resulting products were spliced into a fragment which was packaged into armored RNA for use as a noninfectious, quantifiable synthetic substitute. Furthermore, in the present study we developed a multiplex real-time RT-PCR assay in which the armored RNA was used as an external positive control and the three RNA viruses could be detected simultaneously in a single reaction mix. The detection limit of the multiplex real-time PCR was 10 copies/microl of armored RNA. |*RNA, Viral/analysis/genetics[MESH] |*Reverse Transcriptase Polymerase Chain Reaction/methods/standards[MESH] |Base Sequence[MESH] |Escherichia coli/genetics/metabolism/virology[MESH] |Humans[MESH] |Influenza A virus/genetics/*isolation & purification[MESH] |Influenza B virus/genetics/*isolation & purification[MESH] |Influenza, Human/diagnosis[MESH] |Levivirus/genetics/metabolism[MESH] |Molecular Sequence Data[MESH] |Reference Standards[MESH] |Severe Acute Respiratory Syndrome/diagnosis[MESH] |Severe acute respiratory syndrome-related coronavirus/genetics/*isolation & purification[MESH]Preparation of armored RNA as a control for multiplex real-time revers(epmc).pdf DeepDyve Pubget Overpricing