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10.1152/ajprenal.00245.2010 http://scihub22266oqcxt.onion/10.1152/ajprenal.00245.2010 21123494!3044001!21123494     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\21123494.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Renal+Physiol 2011 ; 300 (2): F412-24 Nephropedia Template TP {{tp|p=21123494|t=2011. Oxygen regulation of the epithelial Na channel in the collecting duct |pdf=|usr=}}{{21123494}} gab.com Text Oxygen regulation of the epithelial Na channel in the collecting duct JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=21123494 #FOAvip The PO(2) within the kidney changes dramatically from cortex to medulla. The present experiments examined the effect of changing PO(2) on epithelial Na channel (ENaC)-mediated Na transport in the collecting duct using the mpkCCD-c14 cell line. Decreasing ambient O(2) concentration from 20 to 8% decreased ENaC activity by 40%; increasing O(2) content to 40% increased ENaC activity by 50%. The O(2) effect required several hours to develop and was not mimicked by the acid pH that developed in monolayers incubated in low-O(2) medium. Corticosteroids increased ENaC activity at each O(2) concentration; there was no interaction. The pathways for O(2) and steroid regulation of ENaC are different since O(2) did not substantially affect Sgk1, alpha-ENaC, Gilz, or Usp2-45 mRNA levels, genes involved in steroid-mediated ENaC regulation. The regulation of ENaC activity by these levels of O(2) appears not to be mediated by changes in hypoxia-inducible factor-1alpha or -2alpha activity or a change in AMP kinase activity. Changes in O(2) concentration had minimal effect on alpha- or gamma-ENaC mRNA and protein levels; there were moderate effects on beta-ENaC levels. However, 40% O(2) induced substantially greater total beta- and gamma-ENaC on the apical surface compared with 8% O(2); both subunits demonstrated a greater increase in the mature forms. The alpha-ENaC subunit was difficult to detect on the apical surface, perhaps because our antibodies do not recognize the major mature form. These results identify a mechanism of ENaC regulation that may be important in different regions of the kidney and in responses to changes in dietary NaCl. Twit Text FOAVip Oxygen regulation of the epithelial Na channel in the collecting duct ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=21123494 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21123494 #FOAvip English Wikipedia <ref>{{Cite pmid|21123494}}</ref> Oxygen regulation of the epithelial Na channel in the collecting duct #MMPMID21123494 Husted RF; Lu H; Sigmund RD; Stokes JB Am J Physiol Renal Physiol 2011[Feb]; 300 (2): F412-24 PMID21123494show ga The PO(2) within the kidney changes dramatically from cortex to medulla. The present experiments examined the effect of changing PO(2) on epithelial Na channel (ENaC)-mediated Na transport in the collecting duct using the mpkCCD-c14 cell line. Decreasing ambient O(2) concentration from 20 to 8% decreased ENaC activity by 40%; increasing O(2) content to 40% increased ENaC activity by 50%. The O(2) effect required several hours to develop and was not mimicked by the acid pH that developed in monolayers incubated in low-O(2) medium. Corticosteroids increased ENaC activity at each O(2) concentration; there was no interaction. The pathways for O(2) and steroid regulation of ENaC are different since O(2) did not substantially affect Sgk1, alpha-ENaC, Gilz, or Usp2-45 mRNA levels, genes involved in steroid-mediated ENaC regulation. The regulation of ENaC activity by these levels of O(2) appears not to be mediated by changes in hypoxia-inducible factor-1alpha or -2alpha activity or a change in AMP kinase activity. Changes in O(2) concentration had minimal effect on alpha- or gamma-ENaC mRNA and protein levels; there were moderate effects on beta-ENaC levels. However, 40% O(2) induced substantially greater total beta- and gamma-ENaC on the apical surface compared with 8% O(2); both subunits demonstrated a greater increase in the mature forms. The alpha-ENaC subunit was difficult to detect on the apical surface, perhaps because our antibodies do not recognize the major mature form. These results identify a mechanism of ENaC regulation that may be important in different regions of the kidney and in responses to changes in dietary NaCl. |Adenylate Kinase/metabolism[MESH] |Adrenal Cortex Hormones/pharmacology[MESH] |Animals[MESH] |Basic Helix-Loop-Helix Transcription Factors/metabolism[MESH] |Cell Line[MESH] |Epithelial Sodium Channels/drug effects/*metabolism[MESH] |Humans[MESH] |Hydrogen-Ion Concentration[MESH] |Hypoxia-Inducible Factor 1/metabolism[MESH] |Kidney Tubules, Collecting/drug effects/*metabolism[MESH] |Mice[MESH]Oxygen regulation of the epithelial Na channel in the collecting duct (epmc).pdf DeepDyve Pubget Overpricing