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10.1016/j.jss.2013.03.034 http://scihub22266oqcxt.onion/10.1016/j.jss.2013.03.034 23628437!ä!23628437 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23628437.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Surg+Res 2013 ; 184 (2): 944-50 Nephropedia Template TP {{tp|p=23628437|t=2013. Magnesium sulfate provides neuroprotection in lipopolysaccharide-activated primary microglia by inhibiting NF-kappaB pathway |pdf=|usr=}}{{23628437}} gab.com Text Magnesium sulfate provides neuroprotection in lipopolysaccharide-activated primary microglia by inhibiting NF-kappaB pathway JO: J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=23628437 #FOAvip BACKGROUND: Magnesium sulfate has been used as an anticonvulsant in severe preeclamptic or eclamptic women prior to surgical trauma, but its effects on neuroinflammation is not well defined. In the present study, we investigated the neuroprotective effects of magnesium sulfate in lipopolysaccharide (LPS)-induced microglia and explored the underlying mechanism. MATERIALS AND METHODS: Microglia was incubated with LPS in the presence or absence of various concentrations of magnesium sulfate, or L-type calcium channel activator BAY-K8644. The levels of inflammatory mediators, such as nitric oxide, prostaglandin E2, interleukin 1beta, and tumor necrosis factor alpha, were measured using enzyme-linked immunosorbent assay. The expression of inducible nitric oxide synthase mRNA was detected by reverse-transcription polymerase chain reaction. Nuclear factor kappaB (NF-kappaB) activity in the nuclear extract of microglia was detected by NF-kappaB p50/p65 transcription factor assay kit. RESULTS: Magnesium sulfate at 5 and 10 mmol/L significantly inhibited the release of nitric oxide, prostaglandin E2, interleukin 1beta, and tumor necrosis factor alpha, and the expression of inducible nitric oxide synthase mRNA in LPS-activated microglia. Furthermore, magnesium sulfate inhibited the translocation of NF-kappaB from the cytoplasm to the nucleus in a dose-dependent manner. Notably, these effects were significantly reversed by L-type calcium channel activator BAY-K8644. CONCLUSIONS: Magnesium sulfate protects microglia against LPS-induced release of inflammatory mediators, and these effects may be mediated by inhibiting L-type calcium channels and NF-kappaB signaling. Twit Text FOAVip Magnesium sulfate provides neuroprotection in lipopolysaccharide-activated primary microglia by inhibiting NF-kappaB pathway ????J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=23628437 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23628437 #FOAvip English Wikipedia <ref>{{Cite pmid|23628437}}</ref> Magnesium sulfate provides neuroprotection in lipopolysaccharide-activated primary microglia by inhibiting NF-kappaB pathway #MMPMID23628437 Gao F; Ding B; Zhou L; Gao X; Guo H; Xu H J Surg Res 2013[Oct]; 184 (2): 944-50 PMID23628437show ga BACKGROUND: Magnesium sulfate has been used as an anticonvulsant in severe preeclamptic or eclamptic women prior to surgical trauma, but its effects on neuroinflammation is not well defined. In the present study, we investigated the neuroprotective effects of magnesium sulfate in lipopolysaccharide (LPS)-induced microglia and explored the underlying mechanism. MATERIALS AND METHODS: Microglia was incubated with LPS in the presence or absence of various concentrations of magnesium sulfate, or L-type calcium channel activator BAY-K8644. The levels of inflammatory mediators, such as nitric oxide, prostaglandin E2, interleukin 1beta, and tumor necrosis factor alpha, were measured using enzyme-linked immunosorbent assay. The expression of inducible nitric oxide synthase mRNA was detected by reverse-transcription polymerase chain reaction. Nuclear factor kappaB (NF-kappaB) activity in the nuclear extract of microglia was detected by NF-kappaB p50/p65 transcription factor assay kit. RESULTS: Magnesium sulfate at 5 and 10 mmol/L significantly inhibited the release of nitric oxide, prostaglandin E2, interleukin 1beta, and tumor necrosis factor alpha, and the expression of inducible nitric oxide synthase mRNA in LPS-activated microglia. Furthermore, magnesium sulfate inhibited the translocation of NF-kappaB from the cytoplasm to the nucleus in a dose-dependent manner. Notably, these effects were significantly reversed by L-type calcium channel activator BAY-K8644. CONCLUSIONS: Magnesium sulfate protects microglia against LPS-induced release of inflammatory mediators, and these effects may be mediated by inhibiting L-type calcium channels and NF-kappaB signaling. |3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology[MESH] |Animals[MESH] |Calcium Channel Agonists/pharmacology[MESH] |Calcium Channels, L-Type/drug effects/physiology[MESH] |Cells, Cultured[MESH] |Dinoprostone/metabolism[MESH] |Dose-Response Relationship, Drug[MESH] |Inflammation/chemically induced/physiopathology/*prevention & control[MESH] |Interleukin-1beta/metabolism[MESH] |Lipopolysaccharides/adverse effects/*pharmacology[MESH] |Magnesium Sulfate/pharmacology/*therapeutic use[MESH] |Microglia/cytology/drug effects/*physiology[MESH] |Models, Animal[MESH] |NF-kappa B/*antagonists & inhibitors/drug effects[MESH] |Neuroprotective Agents/pharmacology/*therapeutic use[MESH] |Nitric Oxide Synthase Type II/metabolism[MESH] |Nitric Oxide/metabolism[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Signal Transduction/*drug effects/physiology[MESH]Magnesium sulfate provides neuroprotection in lipopolysaccharide-activ(epmc).pdf DeepDyve Pubget Overpricing