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10.1016/0014-2999(89)90291-4 http://scihub22266oqcxt.onion/10.1016/0014-2999(89)90291-4 2524398!�!2524398 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\2524398.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Eur+J+Pharmacol 1989 ; 162 (2): 281-8 Nephropedia Template TP {{tp|p=2524398|t=1989. Direct binding of atrial natriuretic factor to adrenocortical mitochondria |pdf=|usr=}}{{2524398}} gab.com Text Direct binding of atrial natriuretic factor to adrenocortical mitochondria JO: Eur J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=2524398 #FOAvip Atrial natriuretic factor (ANF) is a known antagonist of adrenocortical aldosterone synthesis and secretion. Immunocytochemical and ultrastructural autoradiographic evidence suggests that ANF may bind to mitochondria of a number of target tissues including adrenal cortex. Consequently, the ability of [125I]ANF to bind directly to isolated bovine adrenocortical mitochondria was assessed. Mitochondrial-enriched subfractions of adrenocortical homogenates were prepared by differential and sucrose gradient centrifugation. Mitochondrial membranes specifically bound [125I]ANF. At 20 degrees C equilibrium was achieved between 90 and 120 min. [125I]ANF binding was inhibited in a concentration-dependent manner by unlabelled ANF (IC50 about 5 x 10(-10) M); other substances with biological actions on glomerulosa cells (arginine vasopressin, angiotensin II) did not alter [125I]ANF binding. Similarly shorter ANF fragments including ANF-(103-125), ANF-(99-109) and ANF-(111-126) had no significant competitive effect on binding of the labelled ligand. While Ca2+ and Mg2+ had little effect on ANF binding, the divalent cation Ni2+ inhibited binding of radiolabelled ANF by 90% (IC50 about 8.3 x 10(-5) M). Scatchard analysis revealed both high and low affinity binding sites for [125I]ANF with respective KDs of 4.7 +/- 7 pM and 0.3 +/- 0.02 nM and receptor densities of 1.1 +/- 0.2 and 8.6 +/- 0.1 pmol/mg protein. At 0.2 nM, Ni2+ caused a 5-fold and 100-fold decrease in high and low affinity [125I]ANF binding, respectively. The data demonstrate that ANF binds directly to mitochondria and perhaps it is at this site that the atrial peptide negatively modulates agonist-induced aldosterone biosynthesis. Twit Text FOAVip Direct binding of atrial natriuretic factor to adrenocortical mitochondria ????Eur J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=2524398 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2524398 #FOAvip English Wikipedia <ref>{{Cite pmid|2524398}}</ref> Direct binding of atrial natriuretic factor to adrenocortical mitochondria #MMPMID2524398 Heisler S Eur J Pharmacol 1989[Mar]; 162 (2): 281-8 PMID2524398show ga Atrial natriuretic factor (ANF) is a known antagonist of adrenocortical aldosterone synthesis and secretion. Immunocytochemical and ultrastructural autoradiographic evidence suggests that ANF may bind to mitochondria of a number of target tissues including adrenal cortex. Consequently, the ability of [125I]ANF to bind directly to isolated bovine adrenocortical mitochondria was assessed. Mitochondrial-enriched subfractions of adrenocortical homogenates were prepared by differential and sucrose gradient centrifugation. Mitochondrial membranes specifically bound [125I]ANF. At 20 degrees C equilibrium was achieved between 90 and 120 min. [125I]ANF binding was inhibited in a concentration-dependent manner by unlabelled ANF (IC50 about 5 x 10(-10) M); other substances with biological actions on glomerulosa cells (arginine vasopressin, angiotensin II) did not alter [125I]ANF binding. Similarly shorter ANF fragments including ANF-(103-125), ANF-(99-109) and ANF-(111-126) had no significant competitive effect on binding of the labelled ligand. While Ca2+ and Mg2+ had little effect on ANF binding, the divalent cation Ni2+ inhibited binding of radiolabelled ANF by 90% (IC50 about 8.3 x 10(-5) M). Scatchard analysis revealed both high and low affinity binding sites for [125I]ANF with respective KDs of 4.7 +/- 7 pM and 0.3 +/- 0.02 nM and receptor densities of 1.1 +/- 0.2 and 8.6 +/- 0.1 pmol/mg protein. At 0.2 nM, Ni2+ caused a 5-fold and 100-fold decrease in high and low affinity [125I]ANF binding, respectively. The data demonstrate that ANF binds directly to mitochondria and perhaps it is at this site that the atrial peptide negatively modulates agonist-induced aldosterone biosynthesis. |Adrenal Cortex/drug effects/*metabolism[MESH] |Animals[MESH] |Atrial Natriuretic Factor/*metabolism[MESH] |Biomarkers[MESH] |Cattle[MESH] |In Vitro Techniques[MESH] |Iodine Radioisotopes[MESH] |Male[MESH] |Mitochondria/drug effects/*metabolism[MESH] |Nickel/pharmacology[MESH] |Rats[MESH] |Subcellular Fractions/drug effects/enzymology/metabolism[MESH]Direct binding of atrial natriuretic factor to adrenocortical mitochon(epmc).pdf DeepDyve Pubget Overpricing