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10.1126/science.aaa5945 http://scihub22266oqcxt.onion/10.1126/science.aaa5945 25908821!4687737!25908821     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25908821&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25908821.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Science 2015 ; 348 (6233): 442-4 Nephropedia Template TP {{tp|p=25908821|t=2015. Genome editing The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations |pdf=|usr=}}{{25908821}} gab.com Text Genome editing The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms. Twit Text FOAVip Genome editing The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations ????Science http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip English Wikipedia <ref>{{Cite pmid|25908821}}</ref> Genome editing The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations #MMPMID25908821 Gantz VM; Bier E Science 2015[Apr]; 348 (6233): 442-4 PMID25908821show ga An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms. |*Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |*Heterozygote[MESH] |*Homozygote[MESH] |*Mutagenesis[MESH] |Alleles[MESH] |Animals[MESH] |Drosophila melanogaster/genetics[MESH] |Female[MESH] |Genetic Engineering/*methods[MESH] |Genome, Insect[MESH] |Germ Cells[MESH] |Male[MESH] |Mutation[MESH]Genome editing The mutagenic chain reaction: a method for converting (epmc).pdf DeepDyve Pubget Overpricing