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10.2217/fmb-2016-0190 http://scihub22266oqcxt.onion/10.2217/fmb-2016-0190 28594235!ä!28594235 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28594235&cmd=llinks): Failed to open stream: HTTP request failed! 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Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressing immunogenic HA1 protein enhances immunity and protective efficacy against H1N1 influenza virus infection |pdf=|usr=}}{{28594235}} gab.com Text Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressing immunogenic HA1 protein enhances immunity and protective efficacy against H1N1 influenza virus infection JO: Future Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28594235 #FOAvip AIM: To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264.7 cells. The JOL1635 immunized chickens showed the significant increase in HA1-specific IgG, sIgA antibody, IFN-gamma, IL-6 cytokine and cellular immune responses. The postoral challenge, the JOL1635-immunized chickens showed a faster clearance of PR8 virus cloacal shedding than the control group. CONCLUSION: Generated JOL1635 can establish specific immunogenicity and protection against the PR8 virus in chickens. Twit Text FOAVip Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressing immunogenic HA1 protein enhances immunity and protective efficacy against H1N1 influenza virus infection ????Future Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28594235 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28594235 #FOAvip English Wikipedia <ref>{{Cite pmid|28594235}}</ref> Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressing immunogenic HA1 protein enhances immunity and protective efficacy against H1N1 influenza virus infection #MMPMID28594235 Kamble NM; Hyoung KJ; Lee JH Future Microbiol 2017[Jul]; 12 (ä): 739-752 PMID28594235show ga AIM: To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264.7 cells. The JOL1635 immunized chickens showed the significant increase in HA1-specific IgG, sIgA antibody, IFN-gamma, IL-6 cytokine and cellular immune responses. The postoral challenge, the JOL1635-immunized chickens showed a faster clearance of PR8 virus cloacal shedding than the control group. CONCLUSION: Generated JOL1635 can establish specific immunogenicity and protection against the PR8 virus in chickens. |*Immunogenicity, Vaccine[MESH] |Animals[MESH] |Antibodies, Viral/biosynthesis[MESH] |Chickens[MESH] |Genetic Vectors[MESH] |Hemagglutinin Glycoproteins, Influenza Virus/genetics/*immunology[MESH] |Immunity, Cellular[MESH] |Immunoglobulin A/biosynthesis[MESH] |Immunoglobulin G/biosynthesis[MESH] |Influenza A Virus, H1N1 Subtype/*immunology[MESH] |Influenza Vaccines/administration & dosage/genetics/*immunology[MESH] |Influenza in Birds/*immunology/prevention & control[MESH] |Interferon-gamma/biosynthesis[MESH] |Interleukin-6/biosynthesis[MESH] |Mice[MESH] |Mutation[MESH] |RAW 264.7 Cells[MESH] |Salmonella typhimurium/*genetics[MESH]Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressin(epmc).pdf DeepDyve Pubget Overpricing