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10.18632/oncotarget.23598 http://scihub22266oqcxt.onion/10.18632/oncotarget.23598 29435164!5797035!29435164     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29435164.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Oncotarget 2018 ; 9 (4): 5084-5104 Nephropedia Template TP {{tp|p=29435164|t=2018. Overexpression of Na(+)/Mg(2+) exchanger SLC41A1 attenuates pro-survival signaling |pdf=|usr=}}{{29435164}} gab.com Text Overexpression of Na(+)/Mg(2+) exchanger SLC41A1 attenuates pro-survival signaling JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=29435164 #FOAvip The Na(+)/Mg(2+) exchanger SLC41A1 (A1), a key component of intracellular Mg homeostasis (IMH), is the major cellular Mg(2+) efflux system, and its overexpression decreases [Mg(2+)](intracellular). IMH plays an important role in the regulation of many cellular processes, including cellular signaling. However, whether the overexpression of A1 and the consequent drop of [Mg(2+)](i) impact on intracellular signaling is unknown. To examine the latter, we utilized dynamic mass redistribution (DMR) assay, PathScan((R)) RTK signaling antibody (PRSA) array, confirmatory Western blot (WB) analyses of phosphorylation of kinases selected by PRSA, and mag-fura 2-assisted fast filter spectrometry (FFS). We demonstrate here that the overexpression of A1 quantitatively and qualitatively changes the DMR signal evoked by the application of PAR-1-selective activating peptide and/or by changing [Mg(2+)](extracellular) in HEK293 cells. PRSA profiling of the phosphorylation of important signaling nodes followed by confirmatory WB has revealed that, in HEK293 cells, A1 overexpression significantly attenuates the phosphorylation of Akt/PKB on Thr(308) and/or Ser(473) and of Erk1/2 on Thr(202)/Tyr(204) in the presence of 0 or 1 mM (physiological) Mg(2+) in the bath solution. The latter is also true for SH-SY5Y and HeLa cells. Overexpression of A1 in HEK293 cells significantly lowers [Mg(2+)](i) in the presence of [Mg(2+)](e) = 0 or 1 mM. This correlates with the observed attenuation of prosurvival Akt/PKB - Erk1/2 signaling in these cells. Thus, A1 expression status and [Mg(2+)](e) (and consequently also [Mg(2+)](i)) modulate the complex physiological fingerprint of the cell and influence the activity of kinases involved in anti-apoptotic and, hence, pro-survival events in cells. Twit Text FOAVip Overexpression of Na(+)/Mg(2+) exchanger SLC41A1 attenuates pro-survival signaling ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=29435164 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29435164 #FOAvip English Wikipedia <ref>{{Cite pmid|29435164}}</ref> Overexpression of Na(+)/Mg(2+) exchanger SLC41A1 attenuates pro-survival signaling #MMPMID29435164 Sponder G; Abdulhanan N; Frohlich N; Mastrototaro L; Aschenbach JR; Rontgen M; Pilchova I; Cibulka M; Racay P; Kolisek M Oncotarget 2018[Jan]; 9 (4): 5084-5104 PMID29435164show ga The Na(+)/Mg(2+) exchanger SLC41A1 (A1), a key component of intracellular Mg homeostasis (IMH), is the major cellular Mg(2+) efflux system, and its overexpression decreases [Mg(2+)](intracellular). IMH plays an important role in the regulation of many cellular processes, including cellular signaling. However, whether the overexpression of A1 and the consequent drop of [Mg(2+)](i) impact on intracellular signaling is unknown. To examine the latter, we utilized dynamic mass redistribution (DMR) assay, PathScan((R)) RTK signaling antibody (PRSA) array, confirmatory Western blot (WB) analyses of phosphorylation of kinases selected by PRSA, and mag-fura 2-assisted fast filter spectrometry (FFS). We demonstrate here that the overexpression of A1 quantitatively and qualitatively changes the DMR signal evoked by the application of PAR-1-selective activating peptide and/or by changing [Mg(2+)](extracellular) in HEK293 cells. PRSA profiling of the phosphorylation of important signaling nodes followed by confirmatory WB has revealed that, in HEK293 cells, A1 overexpression significantly attenuates the phosphorylation of Akt/PKB on Thr(308) and/or Ser(473) and of Erk1/2 on Thr(202)/Tyr(204) in the presence of 0 or 1 mM (physiological) Mg(2+) in the bath solution. The latter is also true for SH-SY5Y and HeLa cells. Overexpression of A1 in HEK293 cells significantly lowers [Mg(2+)](i) in the presence of [Mg(2+)](e) = 0 or 1 mM. This correlates with the observed attenuation of prosurvival Akt/PKB - Erk1/2 signaling in these cells. Thus, A1 expression status and [Mg(2+)](e) (and consequently also [Mg(2+)](i)) modulate the complex physiological fingerprint of the cell and influence the activity of kinases involved in anti-apoptotic and, hence, pro-survival events in cells. äOverexpression of Na(+)/Mg(2+) exchanger SLC41A1 attenuates pro-surviv(epmc).pdf DeepDyve Pubget Overpricing