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10.1099/jgv.0.001253 http://scihub22266oqcxt.onion/10.1099/jgv.0.001253 30924765!ä!30924765 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30924765&cmd=llinks): Failed to open stream: HTTP request failed! 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Secondary structure of DNA released from purified capsids of human parvovirus B19 under moderate denaturing conditions |pdf=|usr=}}{{30924765}} gab.com Text Secondary structure of DNA released from purified capsids of human parvovirus B19 under moderate denaturing conditions JO: J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=30924765 #FOAvip Parvovirus B19 (B19V) possesses a linear single-stranded DNA genome of either positive or negative polarity. Due to intramolecular sequence homologies, either strand may theoretically be folded in several alternative ways. Viral DNA, when extracted from virions by several procedures, presents as linear single-stranded and/or linear double-stranded molecules, except when one particular commercial kit is used. This protocol yields DNA with an aberrant electrophoretic mobility in addition to linear double-stranded molecules, but never any single-stranded molecules. This peculiar kind of DNA was found in all plasma or serum samples tested and so we decided to analyse its secondary structure. In line with our results for one- and two-dimensional electrophoresis, mobility shift assays, DNA preparation by an in-house extraction method with moderate denaturing conditions, density gradient ultracentrifugation, DNA digestion experiments and competition hybridization assays, we conclude that (i) the unique internal portions of this distinctive single-stranded molecules are folded into tight tangles and (ii) the two terminal redundant regions are associated with each other, yielding non-covalently closed pseudo-circular molecules stabilized by a short (18 nucleotides) intramolecular stem, whereas the extreme 3'- and 5'-ends are folded back on themselves, forming a structure resembling a twin hairpin. The question arises as to whether this fairly unstable structure represents the encapsidated genome structure. The answer to this question remains quite relevant in terms of comprehending the initiation and end of B19V genome replication. Twit Text FOAVip Secondary structure of DNA released from purified capsids of human parvovirus B19 under moderate denaturing conditions ????J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=30924765 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30924765 #FOAvip English Wikipedia <ref>{{Cite pmid|30924765}}</ref> Secondary structure of DNA released from purified capsids of human parvovirus B19 under moderate denaturing conditions #MMPMID30924765 Matz B; Kupfer B; Kallies R; Kulshammer M; Flotenmeyer M; Kreil TR; Eis-Hubinger AM J Gen Virol 2019[May]; 100 (5): 812-827 PMID30924765show ga Parvovirus B19 (B19V) possesses a linear single-stranded DNA genome of either positive or negative polarity. Due to intramolecular sequence homologies, either strand may theoretically be folded in several alternative ways. Viral DNA, when extracted from virions by several procedures, presents as linear single-stranded and/or linear double-stranded molecules, except when one particular commercial kit is used. This protocol yields DNA with an aberrant electrophoretic mobility in addition to linear double-stranded molecules, but never any single-stranded molecules. This peculiar kind of DNA was found in all plasma or serum samples tested and so we decided to analyse its secondary structure. In line with our results for one- and two-dimensional electrophoresis, mobility shift assays, DNA preparation by an in-house extraction method with moderate denaturing conditions, density gradient ultracentrifugation, DNA digestion experiments and competition hybridization assays, we conclude that (i) the unique internal portions of this distinctive single-stranded molecules are folded into tight tangles and (ii) the two terminal redundant regions are associated with each other, yielding non-covalently closed pseudo-circular molecules stabilized by a short (18 nucleotides) intramolecular stem, whereas the extreme 3'- and 5'-ends are folded back on themselves, forming a structure resembling a twin hairpin. The question arises as to whether this fairly unstable structure represents the encapsidated genome structure. The answer to this question remains quite relevant in terms of comprehending the initiation and end of B19V genome replication. |Capsid Proteins/*genetics[MESH] |DNA Replication/genetics[MESH] |DNA, Single-Stranded/genetics[MESH] |DNA, Viral/*genetics[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Nucleic Acid Conformation[MESH] |Parvoviridae Infections/*virology[MESH] |Parvovirus B19, Human/*genetics[MESH]Secondary structure of DNA released from purified capsids of human par(epmc).pdf DeepDyve Pubget Overpricing