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10.1007/978-1-0716-0211-9_2 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_2 31883084!7121971!31883084     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\31883084.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2020 ; 2099 (ä): 9-20 Nephropedia Template TP {{tp|p=31883084|t=2020. Evaluating MERS-CoV Entry Pathways |pdf=|usr=}}{{31883084}} gab.com Text Evaluating MERS-CoV Entry Pathways JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883084 #FOAvip Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging zoonotic pathogen with a broad host range. The extent of MERS-CoV in nature can be traced to its adaptable cell entry steps. The virus can bind host-cell carbohydrates as well as proteinaceous receptors. Following receptor interaction, the virus can utilize diverse host proteases for cleavage activation of virus-host cell membrane fusion and subsequent genome delivery. The fusion and genome delivery steps can be completed at variable times and places, either at or near cell surfaces or deep within endosomes. Investigators focusing on the CoVs have developed several methodologies that effectively distinguish these different cell entry pathways. Here we describe these methods, highlighting virus-cell entry factors, entry inhibitors, and viral determinants that specify the cell entry routes. While the specific methods described herein were utilized to reveal MERS-CoV entry pathways, they are equally suited for other CoVs, as well as other protease-dependent viral species. Twit Text FOAVip Evaluating MERS-CoV Entry Pathways ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883084 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883084 #FOAvip English Wikipedia <ref>{{Cite pmid|31883084}}</ref> Evaluating MERS-CoV Entry Pathways #MMPMID31883084 Qing E; Hantak MP; Galpalli GG; Gallagher T Methods Mol Biol 2020[]; 2099 (ä): 9-20 PMID31883084show ga Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging zoonotic pathogen with a broad host range. The extent of MERS-CoV in nature can be traced to its adaptable cell entry steps. The virus can bind host-cell carbohydrates as well as proteinaceous receptors. Following receptor interaction, the virus can utilize diverse host proteases for cleavage activation of virus-host cell membrane fusion and subsequent genome delivery. The fusion and genome delivery steps can be completed at variable times and places, either at or near cell surfaces or deep within endosomes. Investigators focusing on the CoVs have developed several methodologies that effectively distinguish these different cell entry pathways. Here we describe these methods, highlighting virus-cell entry factors, entry inhibitors, and viral determinants that specify the cell entry routes. While the specific methods described herein were utilized to reveal MERS-CoV entry pathways, they are equally suited for other CoVs, as well as other protease-dependent viral species. |*Virus Internalization[MESH] |Cell Membrane/virology[MESH] |Coronavirus Infections/*virology[MESH] |Endosomes/virology[MESH] |Genome, Viral/*genetics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Membrane Proteins/metabolism[MESH] |Middle East Respiratory Syndrome Coronavirus/genetics/isolation & purification/*physiology[MESH] |Peptide Hydrolases/metabolism[MESH] |RNA-Binding Proteins/metabolism[MESH] |Receptors, Virus/genetics/metabolism[MESH] |Serine Endopeptidases/metabolism[MESH]Evaluating MERS-CoV Entry Pathways (epmc).pdf DeepDyve Pubget Overpricing