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10.1007/978-1-0716-0211-9_5 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_5 31883087!7121842!31883087     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31883087&cmd=llinks): Failed to open stream: HTTP request failed! 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Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV |pdf=|usr=}}{{31883087}} gab.com Text Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883087 #FOAvip Over the past two decades, several coronavirus (CoV) infectious clones have been engineered, allowing for the manipulation of their large viral genomes (~30 kb) using unique reverse genetic systems. These reverse genetic systems include targeted recombination, in vitro ligation, vaccinia virus vectors, and bacterial artificial chromosomes (BACs). Quickly after the identification of Middle East respiratory syndrome-CoV (MERS-CoV), both in vitro ligation and BAC-based reverse genetic technologies were engineered for MERS-CoV to study its basic biological properties, develop live-attenuated vaccines, and test antiviral drugs. Here, I will describe how lambda red recombination can be used with the MERS-CoV BAC to quickly and efficiently introduce virtually any type of genetic modification (point mutations, insertions, deletions) into the MERS-CoV genome and recover recombinant virus. Twit Text FOAVip Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883087 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883087 #FOAvip English Wikipedia <ref>{{Cite pmid|31883087}}</ref> Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV #MMPMID31883087 Fehr AR Methods Mol Biol 2020[]; 2099 (ä): 53-68 PMID31883087show ga Over the past two decades, several coronavirus (CoV) infectious clones have been engineered, allowing for the manipulation of their large viral genomes (~30 kb) using unique reverse genetic systems. These reverse genetic systems include targeted recombination, in vitro ligation, vaccinia virus vectors, and bacterial artificial chromosomes (BACs). Quickly after the identification of Middle East respiratory syndrome-CoV (MERS-CoV), both in vitro ligation and BAC-based reverse genetic technologies were engineered for MERS-CoV to study its basic biological properties, develop live-attenuated vaccines, and test antiviral drugs. Here, I will describe how lambda red recombination can be used with the MERS-CoV BAC to quickly and efficiently introduce virtually any type of genetic modification (point mutations, insertions, deletions) into the MERS-CoV genome and recover recombinant virus. |Bacteriophage lambda/*genetics[MESH] |Chromosomes, Artificial, Bacterial/*genetics[MESH] |Coronavirus Infections/prevention & control/*virology[MESH] |Deoxyribonuclease I/genetics/metabolism[MESH] |Genetic Engineering[MESH] |Genome, Viral/*genetics[MESH] |Homologous Recombination[MESH] |Humans[MESH] |Middle East Respiratory Syndrome Coronavirus/*genetics/immunology[MESH] |Mutation[MESH] |Vaccines, Attenuated/genetics[MESH] |Vaccinia virus/genetics[MESH]Bacterial Artificial Chromosome-Based Lambda Red Recombination with th(epmc).pdf DeepDyve Pubget Overpricing