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10.1007/978-1-0716-0211-9_13 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_13 31883095!7122213!31883095     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\31883095.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2020 ; 2099 (ä): 161-171 Nephropedia Template TP {{tp|p=31883095|t=2020. Development of a Mouse-Adapted MERS Coronavirus |pdf=|usr=}}{{31883095}} gab.com Text Development of a Mouse-Adapted MERS Coronavirus JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883095 #FOAvip First identified in 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel virus that can cause acute respiratory distress syndrome (ARDS), multiorgan failure, and death, with a case fatality rate of ~35%. An animal model that supports MERS-CoV infection and causes severe lung disease is useful to study pathogenesis and evaluate therapies and vaccines. The murine dipeptidyl peptidase 4 (Dpp4) protein is not a functional receptor for MERS-CoV; thus, mice are resistant to MERS-CoV infection. We generated human DPP4 knock-in (hDPP4 KI) mice by replacing exons 10-12 at the mouse Dpp4 locus with exons 10-12 from the human DPP4 gene. The resultant human DPP4 KI mice are permissive to MERS-CoV (HCoV-EMC/2012 strain) infection but develop no disease. To generate a mouse model with associated morbidity and mortality from respiratory disease, we serially passaged HCoV-EMC/2012 strain in the lungs of young hDPP4 KI mice. After 30 in vivo passages, an adapted virus clone was isolated and designated MERS(MA)6.1.2. This virus clone produced significantly higher titers than the parental clone in the lungs of hDPP4 KI mice and caused diffuse lung injury and a fatal respiratory infection. In this chapter, we will describe in detail the procedures used to mouse adapt MERS-CoV by serial passage of the virus in lungs. We also describe the methods used to isolate virus clones and characterize virus infection. Twit Text FOAVip Development of a Mouse-Adapted MERS Coronavirus ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883095 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883095 #FOAvip English Wikipedia <ref>{{Cite pmid|31883095}}</ref> Development of a Mouse-Adapted MERS Coronavirus #MMPMID31883095 Li K; McCray PB Jr Methods Mol Biol 2020[]; 2099 (ä): 161-171 PMID31883095show ga First identified in 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel virus that can cause acute respiratory distress syndrome (ARDS), multiorgan failure, and death, with a case fatality rate of ~35%. An animal model that supports MERS-CoV infection and causes severe lung disease is useful to study pathogenesis and evaluate therapies and vaccines. The murine dipeptidyl peptidase 4 (Dpp4) protein is not a functional receptor for MERS-CoV; thus, mice are resistant to MERS-CoV infection. We generated human DPP4 knock-in (hDPP4 KI) mice by replacing exons 10-12 at the mouse Dpp4 locus with exons 10-12 from the human DPP4 gene. The resultant human DPP4 KI mice are permissive to MERS-CoV (HCoV-EMC/2012 strain) infection but develop no disease. To generate a mouse model with associated morbidity and mortality from respiratory disease, we serially passaged HCoV-EMC/2012 strain in the lungs of young hDPP4 KI mice. After 30 in vivo passages, an adapted virus clone was isolated and designated MERS(MA)6.1.2. This virus clone produced significantly higher titers than the parental clone in the lungs of hDPP4 KI mice and caused diffuse lung injury and a fatal respiratory infection. In this chapter, we will describe in detail the procedures used to mouse adapt MERS-CoV by serial passage of the virus in lungs. We also describe the methods used to isolate virus clones and characterize virus infection. |Animals[MESH] |Coronavirus Infections/*virology[MESH] |Dipeptidyl Peptidase 4/*genetics[MESH] |Disease Models, Animal[MESH] |Humans[MESH] |Lung/virology[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Middle East Respiratory Syndrome Coronavirus/*pathogenicity[MESH] |Respiratory Distress Syndrome/*virology[MESH] |Serial Passage[MESH]Development of a Mouse-Adapted MERS Coronavirus (epmc).pdf DeepDyve Pubget Overpricing