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10.1016/j.cmi.2020.05.007 http://scihub22266oqcxt.onion/10.1016/j.cmi.2020.05.007 32422410!7227500!32422410     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\32422410.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Clin+Microbiol+Infect 2020 ; 26 (8): 1076-1081 Nephropedia Template TP {{tp|p=32422410|t=2020. Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA |pdf=|usr=}}{{32422410}} gab.com Text Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32422410 #FOAvip OBJECTIVE: To evaluate the performance of an ultrafast single-tube nucleic acid isothermal amplification detection assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA using clinical samples from multiple centres. METHODS: A reverse transcription recombinase-aided amplification (RT-RAA) assay for SARS-CoV-2 was conducted within 15 minutes at 39 degrees C with portable instruments after addition of extracted RNA. The clinical performance of RT-RAA assay was evaluated using 947 clinical samples from five institutions in four regions of China; approved commercial fluorescence quantitative real-time PCR (qRT-PCR) kits were used for parallel detection. The sensitivity and specificity of RT-RAA were compared and analysed. RESULTS: The RT-RAA test results of 926 samples were consistent with those of qRT-PCR (330 were positive, 596 negative); 21 results were inconsistent. The sensitivity and specificity of RT-RAA was 97.63% (330/338, 95% confidence interval (CI) 95.21 to 98.90) and 97.87% (596/609, 95% CI 96.28 to 98.81) respectively. The positive and negative predictive values were 96.21% (330/343, 95% CI 93.45 to 97.88) and 98.68% (596/604, 95% CI 97.30 to 99.38) respectively. The total coincidence rate was 97.78% (926/947, 95% CI 96.80 to 98.70), and the kappa was 0.952 (p < 0.05). CONCLUSIONS: With comparable sensitivity and specificity to the commercial qRT-PCR kits, RT-RAA assay for SARS-CoV-2 exhibited the distinctive advantages of simplicity and rapidity in terms of operation and turnaround time. Twit Text FOAVip Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32422410 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32422410 #FOAvip English Wikipedia <ref>{{Cite pmid|32422410}}</ref> Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA #MMPMID32422410 Wang J; Cai K; He X; Shen X; Wang J; Liu J; Xu J; Qiu F; Lei W; Cui L; Ge Y; Wu T; Zhang Y; Yan H; Chen Y; Yu J; Ma X; Shi H; Zhang R; Li X; Gao Y; Niu P; Tan W; Wu G; Jiang Y; Xu W; Ma X Clin Microbiol Infect 2020[Aug]; 26 (8): 1076-1081 PMID32422410show ga OBJECTIVE: To evaluate the performance of an ultrafast single-tube nucleic acid isothermal amplification detection assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA using clinical samples from multiple centres. METHODS: A reverse transcription recombinase-aided amplification (RT-RAA) assay for SARS-CoV-2 was conducted within 15 minutes at 39 degrees C with portable instruments after addition of extracted RNA. The clinical performance of RT-RAA assay was evaluated using 947 clinical samples from five institutions in four regions of China; approved commercial fluorescence quantitative real-time PCR (qRT-PCR) kits were used for parallel detection. The sensitivity and specificity of RT-RAA were compared and analysed. RESULTS: The RT-RAA test results of 926 samples were consistent with those of qRT-PCR (330 were positive, 596 negative); 21 results were inconsistent. The sensitivity and specificity of RT-RAA was 97.63% (330/338, 95% confidence interval (CI) 95.21 to 98.90) and 97.87% (596/609, 95% CI 96.28 to 98.81) respectively. The positive and negative predictive values were 96.21% (330/343, 95% CI 93.45 to 97.88) and 98.68% (596/604, 95% CI 97.30 to 99.38) respectively. The total coincidence rate was 97.78% (926/947, 95% CI 96.80 to 98.70), and the kappa was 0.952 (p < 0.05). CONCLUSIONS: With comparable sensitivity and specificity to the commercial qRT-PCR kits, RT-RAA assay for SARS-CoV-2 exhibited the distinctive advantages of simplicity and rapidity in terms of operation and turnaround time. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Child[MESH] |China[MESH] |Coronavirus Infections/*diagnosis[MESH] |Diagnostic Tests, Routine/instrumentation[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*instrumentation[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Temperature[MESH]Multiple-centre clinical evaluation of an ultrafast single-tube assay (epmc).pdf DeepDyve Pubget Overpricing