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10.1016/j.cca.2020.05.049

http://scihub22266oqcxt.onion/10.1016/j.cca.2020.05.049
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32485155!7261064!32485155
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suck abstract from ncbi


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pmid32485155      Clin+Chim+Acta 2020 ; 509 (ä): 18-21
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  • Comparison of the Elecsys(R) Anti-SARS-CoV-2 immunoassay with the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 antibodies in human plasma #MMPMID32485155
  • Egger M; Bundschuh C; Wiesinger K; Gabriel C; Clodi M; Mueller T; Dieplinger B
  • Clin Chim Acta 2020[Oct]; 509 (ä): 18-21 PMID32485155show ga
  • BACKGROUND: Here, we report on a head-to-head comparison of the fully-automated Elecsys(R) Anti-SARS-CoV-2 immunoassay with the EDI(TM) enzyme linked immunosorbent assays (ELISA) for the detection of SARS-CoV-2 antibodies in human plasma. METHODS: SARS-CoV-2 antibodies were measured with the Elecsys(R) assay and the EDI(TM) ELISAs (IgM and IgG) in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. RESULTS: In COVID-19 patients, the percentage of positive results rose with time from symptom onset, peaking to positivity rates after 15-22 days of 100% for the Elecsys(R) assay, of 94% for the EDI(TM) IgM-ELISA and of 100% for the EDI(TM) IgG ELISA. In the 104 blood samples, the agreement between positive/negative classifications of the Elecsys(R) assay and the EDI(TM) ELISAs (IgM or IgG) was 90%. The false positivity rates in the healthy blood donors and the ICU patients were < 1% for the Elecsys(R) assay and < 3% for the EDI(TM) ELISAs. CONCLUSIONS: Our results indicate a high sensitivity and specificity for the Elecsys(R) assay and an acceptable agreement with the EDI(TM) ELISAs.
  • |*Betacoronavirus/isolation & purification[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19[MESH]
  • |Clinical Laboratory Techniques/*methods/*standards[MESH]
  • |Coronavirus Infections/*blood/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay/methods/standards[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay/methods/standards[MESH]
  • |Male[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*blood/*diagnosis[MESH]
  • |SARS-CoV-2[MESH]


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