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Clinical evaluation of serological IgG antibody response on the Abbott Architect for established SARS-CoV-2 infection #MMPMID32531475
Chew KL; Tan SS; Saw S; Pajarillaga A; Zaine S; Khoo C; Wang W; Tambyah P; Jureen R; Sethi SK
Clin Microbiol Infect 2020[Sep]; 26 (9): 1256.e9-1256.e11 PMID32531475show ga
OBJECTIVE: This study aimed to evaluate the diagnostic performance of the Abbott Architect SARS-CoV-2 IgG assay in COVID-19 patients. METHODS: Residual sera from 177 symptomatic SARS-CoV-2-positive patients and 163 non-COVID-19 patients were tested for antibody with the Abbott SARS-CoV-2 IgG assay (Abbott Diagnostics, Chicago, USA). Clinical records for COVID-19 patients were reviewed to determine the time from onset of clinical illness to testing. RESULTS: Specificity of the assay was 100.0% (95%CI: 97.1-100.0%). The clinical sensitivity of the assay varied depending on time from onset of symptoms, increasing with longer periods from the onset of clinical illness. The clinical sensitivity at =6 days was 8.6% (7/81; 95%CI: 3.8-17.5%), at 7-13 days 43.6% (17/39; 95%CI: 28.2-60.2%), at 14-20 days 84.0% (21/25; 95%CI: 63.1-94.7%), and at >/=21 days 84.4% (27/32; 95%CI: 66.5-94.1%). Clinical sensitivity was higher in the >/=14-day group compared to <14 days. There were no differences between the 14-20-day and >/=21-days groups; the combined clinical sensitivity for these groups (>/=14 days) was 84.2% (49/57; 71.6-92.1%). CONCLUSION: The Abbott SARS-CoV-2 IgG test has high specificity. Clinical sensitivity was limited in the early stages of disease but improved from 14 days after the onset of clinical symptoms.