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10.3390/v12060658 http://scihub22266oqcxt.onion/10.3390/v12060658 32570859!7354546!32570859     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32570859&cmd=llinks): Failed to open stream: HTTP request failed! 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Preliminary Data Related to the Effect of Climacostol Produced by the Freshwater Ciliate Climacostomum virens on Human Adenovirus |pdf=|usr=}}{{32570859}} gab.com Text Preliminary Data Related to the Effect of Climacostol Produced by the Freshwater Ciliate Climacostomum virens on Human Adenovirus JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32570859 #FOAvip The new epidemiological scenario has so far focused on the environmental circulation of human viral pathogens. Owing to the side effects of chemical disinfectants, there is an increasing need for knowledge on the use of virucidal compounds, especially those of a natural origin. Climacostol is a molecule produced by a freshwater ciliate and it exhibits activity against bacterial and fungal pathogens. We thus also speculated that there might be an effect on viral viability, which has never been tested. To evaluate such activity, we chose human adenovirus (HAdV), which is representative of waterborne viruses. We conducted experiments using HAdV serotype 5, whose titer was determined by infecting HeLa cell cultures. HAdV5 was shown to be sensitive to climacostol at a concentration of 0.0002 mg/mL, with an approximate 3 Log(10) reduction when the initial titer of HAdV5 was approximately 10(4) and 10(3) TCID(50)/mL. These preliminary results could be an important starting point for further research aimed at improving the characterization of climacostol activity under different experimental conditions and against various viruses, including enveloped ones (i.e., the coronavirus). The production of climacostol by a protist living in fresh water also suggests a possible application in the activated sludge of wastewater treatment plants. Twit Text FOAVip Preliminary Data Related to the Effect of Climacostol Produced by the Freshwater Ciliate Climacostomum virens on Human Adenovirus ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32570859 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32570859 #FOAvip English Wikipedia <ref>{{Cite pmid|32570859}}</ref> Preliminary Data Related to the Effect of Climacostol Produced by the Freshwater Ciliate Climacostomum virens on Human Adenovirus #MMPMID32570859 Verani M; Di Giuseppe G; Federigi I; Buonanno F; Ortenzi C; Carducci A Viruses 2020[Jun]; 12 (6): � PMID32570859show ga The new epidemiological scenario has so far focused on the environmental circulation of human viral pathogens. Owing to the side effects of chemical disinfectants, there is an increasing need for knowledge on the use of virucidal compounds, especially those of a natural origin. Climacostol is a molecule produced by a freshwater ciliate and it exhibits activity against bacterial and fungal pathogens. We thus also speculated that there might be an effect on viral viability, which has never been tested. To evaluate such activity, we chose human adenovirus (HAdV), which is representative of waterborne viruses. We conducted experiments using HAdV serotype 5, whose titer was determined by infecting HeLa cell cultures. HAdV5 was shown to be sensitive to climacostol at a concentration of 0.0002 mg/mL, with an approximate 3 Log(10) reduction when the initial titer of HAdV5 was approximately 10(4) and 10(3) TCID(50)/mL. These preliminary results could be an important starting point for further research aimed at improving the characterization of climacostol activity under different experimental conditions and against various viruses, including enveloped ones (i.e., the coronavirus). The production of climacostol by a protist living in fresh water also suggests a possible application in the activated sludge of wastewater treatment plants. |Adenoviruses, Human/*drug effects[MESH] |Antiviral Agents/*pharmacology[MESH] |Cell Line[MESH] |Ciliophora/metabolism[MESH] |Disinfectants/*pharmacology[MESH] |HeLa Cells[MESH] |Humans[MESH] |Preliminary Data[MESH] |Resorcinols/*pharmacology[MESH] |Sewage/virology[MESH]Preliminary Data Related to the Effect of Climacostol Produced by the (epmc).pdf DeepDyve Pubget Overpricing