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10.1007/978-1-0716-0900-2_6 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_6 32833205!ä!32833205 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\32833205.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2020 ; 2203 (ä): 77-88 Nephropedia Template TP {{tp|p=32833205|t=2020. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study |pdf=|usr=}}{{32833205}} gab.com Text Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833205 #FOAvip Porcine deltacoronavirus (PDCoV) has emerged as a novel, contagious swine enteric coronavirus that causes watery diarrhea and/or vomiting and intestinal villous atrophy in nursing piglets. PDCoV-related diarrhea first occurred in the USA in 2014 and was subsequently reported in South Korea, China, Thailand, Vietnam, and Lao People's Democratic Republic, leading to massive economic losses and posing a threat to the swine industry worldwide. Currently, no treatments or vaccines for PDCoV are available. The critical step in the development of potential vaccines against PDCoV infection is the isolation and propagation of PDCoV in cell culture. This chapter provides a detailed protocol for isolation and propagation of PDCoV in swine testicular (ST) and LLC porcine kidney (LLC-PK) cell cultures supplemented with pancreatin and trypsin, respectively. Filtered clinical samples (swine intestinal contents or feces) applied to ST or LLC-PK cells produce cytopathic effects characterized by rounding, clumping, and detachment of cells. PDCoV replication in cells can be quantifiably monitored by qRT-PCR, immunofluorescence assays, and immune-electron microscopy. Infectious viral titers can be evaluated by using plaque assays or 50% tissue culture infectious dose (TCID(50)) assays. The ST or LLC-PK cells efficiently supported serial passage and propagation of PDCoV. After serial passage of PDCoV in either ST or LLC-PK cells, the virus can be purified further in ST cells by plaque assays. Twit Text FOAVip Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833205 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32833205 #FOAvip English Wikipedia <ref>{{Cite pmid|32833205}}</ref> Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study #MMPMID32833205 Hu H; Jung K; Kenney SP; Saif LJ Methods Mol Biol 2020[]; 2203 (ä): 77-88 PMID32833205show ga Porcine deltacoronavirus (PDCoV) has emerged as a novel, contagious swine enteric coronavirus that causes watery diarrhea and/or vomiting and intestinal villous atrophy in nursing piglets. PDCoV-related diarrhea first occurred in the USA in 2014 and was subsequently reported in South Korea, China, Thailand, Vietnam, and Lao People's Democratic Republic, leading to massive economic losses and posing a threat to the swine industry worldwide. Currently, no treatments or vaccines for PDCoV are available. The critical step in the development of potential vaccines against PDCoV infection is the isolation and propagation of PDCoV in cell culture. This chapter provides a detailed protocol for isolation and propagation of PDCoV in swine testicular (ST) and LLC porcine kidney (LLC-PK) cell cultures supplemented with pancreatin and trypsin, respectively. Filtered clinical samples (swine intestinal contents or feces) applied to ST or LLC-PK cells produce cytopathic effects characterized by rounding, clumping, and detachment of cells. PDCoV replication in cells can be quantifiably monitored by qRT-PCR, immunofluorescence assays, and immune-electron microscopy. Infectious viral titers can be evaluated by using plaque assays or 50% tissue culture infectious dose (TCID(50)) assays. The ST or LLC-PK cells efficiently supported serial passage and propagation of PDCoV. After serial passage of PDCoV in either ST or LLC-PK cells, the virus can be purified further in ST cells by plaque assays. |Animals[MESH] |Cells, Cultured[MESH] |Coronavirus Infections/veterinary/virology[MESH] |Coronavirus/*isolation & purification[MESH] |Feces/virology[MESH] |Serial Passage[MESH] |Swine[MESH] |Swine Diseases/*virology[MESH]Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A(epmc).pdf DeepDyve Pubget Overpricing