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10.1007/s12223-020-00834-0 http://scihub22266oqcxt.onion/10.1007/s12223-020-00834-0 33159654!7648221!33159654     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33159654&cmd=llinks): Failed to open stream: HTTP request failed! 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Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR |pdf=|usr=}}{{33159654}} gab.com Text Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR JO: Folia Microbiol (Praha) http://www.kidney.de/pget.php?&tw=1&pmid=33159654 #FOAvip Pleural and peritoneal infections cause substantial morbidity and mortality. Traditional diagnostic methods rely on the cultivation of clinical samples, which usually takes days to obtain report and holds a low detection sensitivity. In this study, we evaluated a 5-fluorescent-channel droplet digital PCR (ddPCR) system and 5 assay panels for culture-independent rapid pathogen detections directly from pleural and peritoneal fluid samples. Traditional culture of the same sample was used as reference. A total of 40 pleural fluid samples and 19 peritoneal fluid samples were tested in this study. Twenty-five positives including 4 polymicrobial infections by culture and 26 positives including 11 polymicrobial infections by ddPCR were detected for pleural fluid samples; 14 positives including 2 polymicrobial infections by culture and 15 positives including 3 polymicrobial infections by ddPCR were detected for peritoneal fluid samples. Klebsiella pneumoniae was the most common bacterium detected both in pleural and in peritoneal fluid samples. The sensitivity of the ddPCR assay for pleural and peritoneal fluid samples was 96% (95% confidence interval (CI) = 79.65 to 99.90%) and 92.86% (95% CI = 66.13 to 99.82%), respectively. The turnaround time of the ddPCR assay was approximately 3 h comparing with 38.30 +/- 22.44 h for culture-based identifications. Our results demonstrated that the ddPCR assay is a rapid and sensitive method for identifying pathogens responsible for pleural and peritoneal infections and would be a promising approach for early diagnosis and optimizing treatment of infections. Twit Text FOAVip Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR ????Folia Microbiol (Praha) http://www.kidney.de/pget.php?&tw=1&pmid=33159654 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33159654 #FOAvip English Wikipedia <ref>{{Cite pmid|33159654}}</ref> Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR #MMPMID33159654 Zhou F; Sun S; Sun X; Chen Y; Yang X Folia Microbiol (Praha) 2021[Apr]; 66 (2): 213-219 PMID33159654show ga Pleural and peritoneal infections cause substantial morbidity and mortality. Traditional diagnostic methods rely on the cultivation of clinical samples, which usually takes days to obtain report and holds a low detection sensitivity. In this study, we evaluated a 5-fluorescent-channel droplet digital PCR (ddPCR) system and 5 assay panels for culture-independent rapid pathogen detections directly from pleural and peritoneal fluid samples. Traditional culture of the same sample was used as reference. A total of 40 pleural fluid samples and 19 peritoneal fluid samples were tested in this study. Twenty-five positives including 4 polymicrobial infections by culture and 26 positives including 11 polymicrobial infections by ddPCR were detected for pleural fluid samples; 14 positives including 2 polymicrobial infections by culture and 15 positives including 3 polymicrobial infections by ddPCR were detected for peritoneal fluid samples. Klebsiella pneumoniae was the most common bacterium detected both in pleural and in peritoneal fluid samples. The sensitivity of the ddPCR assay for pleural and peritoneal fluid samples was 96% (95% confidence interval (CI) = 79.65 to 99.90%) and 92.86% (95% CI = 66.13 to 99.82%), respectively. The turnaround time of the ddPCR assay was approximately 3 h comparing with 38.30 +/- 22.44 h for culture-based identifications. Our results demonstrated that the ddPCR assay is a rapid and sensitive method for identifying pathogens responsible for pleural and peritoneal infections and would be a promising approach for early diagnosis and optimizing treatment of infections. |*Real-Time Polymerase Chain Reaction[MESH] |Humans[MESH] |Polymerase Chain Reaction[MESH]Rapid and sensitive identification of pleural and peritoneal infection(epmc).pdf DeepDyve Pubget Overpricing