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10.1016/j.mcp.2020.101669 http://scihub22266oqcxt.onion/10.1016/j.mcp.2020.101669 33203619!7581357!33203619     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33203619&cmd=llinks): Failed to open stream: HTTP request failed! 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Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus |pdf=|usr=}}{{33203619}} gab.com Text Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus JO: Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=33203619 #FOAvip Feline coronavirus (FCoV) is classified into two pathotypes: the avirulent feline enteric coronavirus (FECV), and the virulent feline infectious peritonitis virus (FIPV). Rapid pathogen detection, which is efficient and convenient, is the best approach for early confirmatory diagnosis. In this study, we first developed and evaluated a rapid recombinase polymerase amplification (RPA) detection method for FCoV that can detect FCoV within 15 min at 39 degrees C. The detection limit of that assay was 233 copies/muL DNA molecules per reaction. The specificity was high: it did not cross-react with canine distemper virus (CDV), canine coronavirus (CCoV), canine adenovirus (CAV), feline calicivirus (FCV), feline herpesvirus (FHV), or feline parvovirus (FPV). This assay was evaluated using 42 clinical samples (30 diarrhea samples and 12 ascites samples). The coincidence rate between FCoV-RPA and RT-qPCR for detection in clinical samples was 95.2%. In summary, FCoV-RPA analysis provides an efficient, rapid, and sensitive detection method for FCoV. Twit Text FOAVip Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus ????Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=33203619 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33203619 #FOAvip English Wikipedia <ref>{{Cite pmid|33203619}}</ref> Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus #MMPMID33203619 Hu X; Xiao L; Cong X; Zhu Y; Huang B; Cong F Mol Cell Probes 2020[Dec]; 54 (�): 101669 PMID33203619show ga Feline coronavirus (FCoV) is classified into two pathotypes: the avirulent feline enteric coronavirus (FECV), and the virulent feline infectious peritonitis virus (FIPV). Rapid pathogen detection, which is efficient and convenient, is the best approach for early confirmatory diagnosis. In this study, we first developed and evaluated a rapid recombinase polymerase amplification (RPA) detection method for FCoV that can detect FCoV within 15 min at 39 degrees C. The detection limit of that assay was 233 copies/muL DNA molecules per reaction. The specificity was high: it did not cross-react with canine distemper virus (CDV), canine coronavirus (CCoV), canine adenovirus (CAV), feline calicivirus (FCV), feline herpesvirus (FHV), or feline parvovirus (FPV). This assay was evaluated using 42 clinical samples (30 diarrhea samples and 12 ascites samples). The coincidence rate between FCoV-RPA and RT-qPCR for detection in clinical samples was 95.2%. In summary, FCoV-RPA analysis provides an efficient, rapid, and sensitive detection method for FCoV. |Animals[MESH] |Cat Diseases/diagnosis/virology[MESH] |Cats[MESH] |Coronavirus Infections/*diagnosis[MESH] |Coronavirus, Feline/*genetics/isolation & purification[MESH] |Feline Infectious Peritonitis/*diagnosis[MESH] |Molecular Diagnostic Techniques/methods/*veterinary[MESH] |Nucleic Acid Amplification Techniques/*methods/veterinary[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH]Development of a recombinase polymerase amplification fluorescence ass(epmc).pdf DeepDyve Pubget Overpricing