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10.1016/j.jviromet.2020.114056 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114056 33359615!ä!33359615 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\33359615.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Virol+Methods 2021 ; 289 (ä): 114056 Nephropedia Template TP {{tp|p=33359615|t=2021. Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus |pdf=|usr=}}{{33359615}} gab.com Text Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33359615 #FOAvip Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens. Twit Text FOAVip Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33359615 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33359615 #FOAvip English Wikipedia <ref>{{Cite pmid|33359615}}</ref> Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus #MMPMID33359615 Orlova OV; Glazkova DV; Tsyganova GM; Antoshkina IV; Mintaev RR; Tikhonov AS; Bogoslovskaya EV; Shipulin GA J Virol Methods 2021[Mar]; 289 (ä): 114056 PMID33359615show ga Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens. |*Antigens, Viral[MESH] |*Genetic Vectors[MESH] |Animals[MESH] |Cell Line[MESH] |Humans[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/*genetics[MESH]Application of real-time PCR to significantly reduce the time to obtai(epmc).pdf DeepDyve Pubget Overpricing