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10.1016/j.ymthe.2021.04.001 http://scihub22266oqcxt.onion/10.1016/j.ymthe.2021.04.001 33823303!8019652!33823303     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33823303&cmd=llinks): Failed to open stream: HTTP request failed! 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A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice |pdf=|usr=}}{{33823303}} gab.com Text A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice JO: Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=33823303 #FOAvip A self-transcribing and replicating RNA (STARR)-based vaccine (LUNAR-COV19) has been developed to prevent SARS-CoV-2 infection. The vaccine encodes an alphavirus-based replicon and the SARS-CoV-2 full-length spike glycoprotein. Translation of the replicon produces a replicase complex that amplifies and prolongs SARS-CoV-2 spike glycoprotein expression. A single prime vaccination in mice led to robust antibody responses, with neutralizing antibody titers increasing up to day 60. Activation of cell-mediated immunity produced a strong viral antigen-specific CD8(+) T lymphocyte response. Assaying for intracellular cytokine staining for interferon (IFN)gamma and interleukin-4 (IL-4)-positive CD4(+) T helper (Th) lymphocytes as well as anti-spike glycoprotein immunoglobulin G (IgG)2a/IgG1 ratios supported a strong Th1-dominant immune response. Finally, single LUNAR-COV19 vaccination at both 2 mug and 10 mug doses completely protected human ACE2 transgenic mice from both mortality and even measurable infection following wild-type SARS-CoV-2 challenge. Our findings collectively suggest the potential of LUNAR-COV19 as a single-dose vaccine. Twit Text FOAVip A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice ????Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=33823303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33823303 #FOAvip English Wikipedia <ref>{{Cite pmid|33823303}}</ref> A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice #MMPMID33823303 de Alwis R; Gan ES; Chen S; Leong YS; Tan HC; Zhang SL; Yau C; Low JGH; Kalimuddin S; Matsuda D; Allen EC; Hartman P; Park KJ; Alayyoubi M; Bhaskaran H; Dukanovic A; Bao Y; Clemente B; Vega J; Roberts S; Gonzalez JA; Sablad M; Yelin R; Taylor W; Tachikawa K; Parker S; Karmali P; Davis J; Sullivan BM; Sullivan SM; Hughes SG; Chivukula P; Ooi EE Mol Ther 2021[Jun]; 29 (6): 1970-1983 PMID33823303show ga A self-transcribing and replicating RNA (STARR)-based vaccine (LUNAR-COV19) has been developed to prevent SARS-CoV-2 infection. The vaccine encodes an alphavirus-based replicon and the SARS-CoV-2 full-length spike glycoprotein. Translation of the replicon produces a replicase complex that amplifies and prolongs SARS-CoV-2 spike glycoprotein expression. A single prime vaccination in mice led to robust antibody responses, with neutralizing antibody titers increasing up to day 60. Activation of cell-mediated immunity produced a strong viral antigen-specific CD8(+) T lymphocyte response. Assaying for intracellular cytokine staining for interferon (IFN)gamma and interleukin-4 (IL-4)-positive CD4(+) T helper (Th) lymphocytes as well as anti-spike glycoprotein immunoglobulin G (IgG)2a/IgG1 ratios supported a strong Th1-dominant immune response. Finally, single LUNAR-COV19 vaccination at both 2 mug and 10 mug doses completely protected human ACE2 transgenic mice from both mortality and even measurable infection following wild-type SARS-CoV-2 challenge. Our findings collectively suggest the potential of LUNAR-COV19 as a single-dose vaccine. |Alphavirus/genetics/immunology[MESH] |Angiotensin-Converting Enzyme 2/genetics/immunology[MESH] |Animals[MESH] |Antibodies, Neutralizing/*biosynthesis[MESH] |Antibodies, Viral/*biosynthesis[MESH] |CD8-Positive T-Lymphocytes/drug effects/immunology/virology[MESH] |COVID-19 Vaccines/*administration & dosage/biosynthesis/genetics/immunology[MESH] |COVID-19/immunology/pathology/*prevention & control/virology[MESH] |Female[MESH] |Gene Expression[MESH] |Humans[MESH] |Immunity, Cellular/drug effects[MESH] |Immunity, Humoral/drug effects[MESH] |Interferon-gamma/genetics/immunology[MESH] |Interleukin-4/genetics/immunology[MESH] |Mice[MESH] |Mice, Transgenic[MESH] |Replicon/immunology[MESH] |SARS-CoV-2/*drug effects/immunology/pathogenicity[MESH] |Spike Glycoprotein, Coronavirus/chemistry/genetics/*immunology[MESH] |Th1 Cells/drug effects/immunology/virology[MESH] |Transgenes[MESH] |Treatment Outcome[MESH] |Vaccination/methods[MESH] |Vaccines, Synthetic/*administration & dosage/biosynthesis/genetics/immunology[MESH]A single dose of self-transcribing and replicating RNA-based SARS-CoV-(epmc).pdf DeepDyve Pubget Overpricing