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10.3390/v13040603 http://scihub22266oqcxt.onion/10.3390/v13040603 33916100!8066160!33916100     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33916100&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\33916100.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Viruses 2021 ; 13 (4): ä Nephropedia Template TP {{tp|p=33916100|t=2021. Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell Fusion |pdf=|usr=}}{{33916100}} gab.com Text Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell Fusion JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33916100 #FOAvip Sindbis virus (SINV), a positive-sense single stranded RNA virus that causes mild symptoms in humans, is transmitted by mosquito bites. SINV reverse genetics have many implications, not only in understanding alphavirus transmission, replication cycle, and virus-host interactions, but also in biotechnology and biomedical applications. The rescue of SINV infectious particles is usually achieved by transfecting susceptible cells (BHK-21) with SINV-infectious mRNA genomes generated from cDNA constructed via in vitro translation (IVT). That procedure is time consuming, costly, and relies heavily on reagent quality. Here, we constructed a novel infectious SINV cDNA construct that expresses its genomic RNA in yeast cells controlled by galactose induction. Using spheroplasts made from this yeast, we established a robust polyethylene glycol-mediated yeast: BHK-21 fusion protocol to rescue infectious SINV particles. Our approach is timesaving and utilizes common lab reagents for SINV rescue. It could be a useful tool for the rescue of large single strand RNA viruses, such as SARS-CoV-2. Twit Text FOAVip Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell Fusion ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33916100 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33916100 #FOAvip English Wikipedia <ref>{{Cite pmid|33916100}}</ref> Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell Fusion #MMPMID33916100 Ding L; Brown DM; Glass JI Viruses 2021[Apr]; 13 (4): ä PMID33916100show ga Sindbis virus (SINV), a positive-sense single stranded RNA virus that causes mild symptoms in humans, is transmitted by mosquito bites. SINV reverse genetics have many implications, not only in understanding alphavirus transmission, replication cycle, and virus-host interactions, but also in biotechnology and biomedical applications. The rescue of SINV infectious particles is usually achieved by transfecting susceptible cells (BHK-21) with SINV-infectious mRNA genomes generated from cDNA constructed via in vitro translation (IVT). That procedure is time consuming, costly, and relies heavily on reagent quality. Here, we constructed a novel infectious SINV cDNA construct that expresses its genomic RNA in yeast cells controlled by galactose induction. Using spheroplasts made from this yeast, we established a robust polyethylene glycol-mediated yeast: BHK-21 fusion protocol to rescue infectious SINV particles. Our approach is timesaving and utilizes common lab reagents for SINV rescue. It could be a useful tool for the rescue of large single strand RNA viruses, such as SARS-CoV-2. |*Cell Fusion[MESH] |*Spheroplasts[MESH] |Alphavirus Infections/*virology[MESH] |Animals[MESH] |COVID-19[MESH] |DNA, Complementary[MESH] |Host Microbial Interactions/*physiology[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2[MESH] |Saccharomyces cerevisiae[MESH] |Sindbis Virus/*genetics[MESH]Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell(epmc).pdf DeepDyve Pubget Overpricing