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10.1016/j.biomaterials.2021.120876 http://scihub22266oqcxt.onion/10.1016/j.biomaterials.2021.120876 34034027!ä!34034027 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34034027.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biomaterials 2021 ; 274 (ä): 120876 Nephropedia Template TP {{tp|p=34034027|t=2021. Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids |pdf=|usr=}}{{34034027}} gab.com Text Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids JO: Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=34034027 #FOAvip Rapid diagnostics of adventitious agents in biopharmaceutical/cell manufacturing release testing and the fight against viral infection have become critical. Quantitative real-time PCR and CRISPR-based methods rapidly detect DNA/RNA in 1 h but suffer from inter-site variability. Absolute quantification of DNA/RNA by methods such as digital PCR reduce this variability but are currently too slow for wider application. Here, we report a RApid DIgital Crispr Approach (RADICA) for absolute quantification of nucleic acids in 40-60 min. Using SARS-CoV-2 as a proof-of-concept target, RADICA allows for absolute quantification with a linear dynamic range of 0.6-2027 copies/muL (R(2) value > 0.99), high accuracy and low variability, no cross-reactivity to similar targets, and high tolerance to human background DNA. RADICA's versatility is validated against other targets such as Epstein-Barr virus (EBV) from human B cells and patients' serum. RADICA can accurately detect and absolutely quantify EBV DNA with similar dynamic range of 0.5-2100 copies/muL (R(2) value > 0.98) in 1 h without thermal cycling, providing a 4-fold faster alternative to digital PCR-based detection. RADICA therefore enables rapid and sensitive absolute quantification of nucleic acids which can be widely applied across clinical, research, and biomanufacturing areas. Twit Text FOAVip Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids ????Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=34034027 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34034027 #FOAvip English Wikipedia <ref>{{Cite pmid|34034027}}</ref> Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids #MMPMID34034027 Wu X; Tay JK; Goh CK; Chan C; Lee YH; Springs SL; Wang Y; Loh KS; Lu TK; Yu H Biomaterials 2021[Jul]; 274 (ä): 120876 PMID34034027show ga Rapid diagnostics of adventitious agents in biopharmaceutical/cell manufacturing release testing and the fight against viral infection have become critical. Quantitative real-time PCR and CRISPR-based methods rapidly detect DNA/RNA in 1 h but suffer from inter-site variability. Absolute quantification of DNA/RNA by methods such as digital PCR reduce this variability but are currently too slow for wider application. Here, we report a RApid DIgital Crispr Approach (RADICA) for absolute quantification of nucleic acids in 40-60 min. Using SARS-CoV-2 as a proof-of-concept target, RADICA allows for absolute quantification with a linear dynamic range of 0.6-2027 copies/muL (R(2) value > 0.99), high accuracy and low variability, no cross-reactivity to similar targets, and high tolerance to human background DNA. RADICA's versatility is validated against other targets such as Epstein-Barr virus (EBV) from human B cells and patients' serum. RADICA can accurately detect and absolutely quantify EBV DNA with similar dynamic range of 0.5-2100 copies/muL (R(2) value > 0.98) in 1 h without thermal cycling, providing a 4-fold faster alternative to digital PCR-based detection. RADICA therefore enables rapid and sensitive absolute quantification of nucleic acids which can be widely applied across clinical, research, and biomanufacturing areas. |*COVID-19[MESH] |*Epstein-Barr Virus Infections[MESH] |*Nucleic Acids[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Herpesvirus 4, Human/genetics[MESH] |Humans[MESH] |SARS-CoV-2[MESH]Digital CRISPR-based method for the rapid detection and absolute quant(epmc).pdf DeepDyve Pubget Overpricing