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10.1038/s41596-021-00554-w http://scihub22266oqcxt.onion/10.1038/s41596-021-00554-w 34035500!�!34035500 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34035500&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34035500.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Protoc 2021 ; 16 (7): 3639-3671 Nephropedia Template TP {{tp|p=34035500|t=2021. Effective high-throughput isolation of fully human antibodies targeting infectious pathogens |pdf=|usr=}}{{34035500}} gab.com Text Effective high-throughput isolation of fully human antibodies targeting infectious pathogens JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34035500 #FOAvip As exemplified by the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there is a strong demand for rapid high-throughput isolation pipelines to identify potent neutralizing antibodies for prevention and therapy of infectious diseases. However, despite substantial progress and extensive efforts, the identification and production of antigen-specific antibodies remains labor- and cost-intensive. We have advanced existing concepts to develop a highly efficient high-throughput protocol with proven application for the isolation of potent antigen-specific antibodies against human immunodeficiency virus 1, hepatitis C virus, human cytomegalovirus, Middle East respiratory syndrome coronavirus, SARS-CoV-2 and Ebola virus. It is based on computationally optimized multiplex primer sets (openPrimeR), which guarantee high coverage of even highly mutated immunoglobulin gene segments as well as on optimized antibody cloning and production strategies. Here, we provide the detailed protocol, which covers all critical steps from sample collection to antibody production within 12-14 d. Twit Text FOAVip Effective high-throughput isolation of fully human antibodies targeting infectious pathogens ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34035500 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34035500 #FOAvip English Wikipedia <ref>{{Cite pmid|34035500}}</ref> Effective high-throughput isolation of fully human antibodies targeting infectious pathogens #MMPMID34035500 Gieselmann L; Kreer C; Ercanoglu MS; Lehnen N; Zehner M; Schommers P; Potthoff J; Gruell H; Klein F Nat Protoc 2021[Jul]; 16 (7): 3639-3671 PMID34035500show ga As exemplified by the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there is a strong demand for rapid high-throughput isolation pipelines to identify potent neutralizing antibodies for prevention and therapy of infectious diseases. However, despite substantial progress and extensive efforts, the identification and production of antigen-specific antibodies remains labor- and cost-intensive. We have advanced existing concepts to develop a highly efficient high-throughput protocol with proven application for the isolation of potent antigen-specific antibodies against human immunodeficiency virus 1, hepatitis C virus, human cytomegalovirus, Middle East respiratory syndrome coronavirus, SARS-CoV-2 and Ebola virus. It is based on computationally optimized multiplex primer sets (openPrimeR), which guarantee high coverage of even highly mutated immunoglobulin gene segments as well as on optimized antibody cloning and production strategies. Here, we provide the detailed protocol, which covers all critical steps from sample collection to antibody production within 12-14 d. |Antibodies, Monoclonal/immunology/pharmacology[MESH] |Antibodies, Neutralizing/immunology/*isolation & purification/pharmacology[MESH] |Antibodies, Viral/immunology/isolation & purification[MESH] |COVID-19/*immunology[MESH] |Communicable Diseases[MESH] |High-Throughput Screening Assays/*methods[MESH] |Humans[MESH] |Immunoglobulin G/immunology[MESH] |Pandemics[MESH]Effective high-throughput isolation of fully human antibodies targetin(epmc).pdf DeepDyve Pubget Overpricing