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10.3390/v13050818 http://scihub22266oqcxt.onion/10.3390/v13050818 34062916!8147338!34062916     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34062916&cmd=llinks): Failed to open stream: HTTP request failed! 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Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR?|pdf=|usr=}}{{34062916}} gab.com Text Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=34062916 #FOAvip To complement RT-qPCR testing for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many countries have introduced the use of rapid antigen tests. As they generally display lower real-life performances than expected, their correct positioning as frontline screening is still controversial. Despite the lack of data from daily clinical use, third generation microfluidic assays (such as the LumiraDx SARS-CoV-2 Ag test) have recently been suggested to have similar performances to RT-qPCR and have been proposed as alternative diagnostic tools. By analyzing 960 nasopharyngeal swabs from 960 subjects at the emergency department admissions of a tertiary COVID-19 hospital, LumiraDx assay demonstrated a specificity of 97% (95% CI: 96-98), and a sensitivity of 85% (95% CI: 82-89) in comparison with RT-qPCR, which increases to 91% (95% CI: 86-95) for samples with a cycle threshold </= 29. Fifty false-negative LumiraDx-results were confirmed by direct quantification of genomic SARS-CoV-2 RNA through droplet-digital PCR (median (IQR) load = 5880 (1657-41,440) copies/mL). Subgenomic N and E RNAs were detected in 52% (n = 26) and 56% (n = 28) of them, respectively, supporting the presence of active viral replication. Overall, the LumiraDx test complies with the minimum performance requirements of the WHO. Yet, the risk of a misrecognition of patients with active COVID-19 persists, and the need for confirmatory RT-qPCR should not be amended. Twit Text FOAVip Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=34062916 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34062916 #FOAvip English Wikipedia <ref>{{Cite pmid|34062916}}</ref> Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR? #MMPMID34062916 Cento V; Renica S; Matarazzo E; Antonello M; Colagrossi L; Di Ruscio F; Pani A; Fanti D; Vismara C; Puoti M; Scaglione F; Perno CF; Alteri C; On Behalf Of The S Co Va Study Group Viruses 2021[May]; 13 (5): ä PMID34062916show ga To complement RT-qPCR testing for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many countries have introduced the use of rapid antigen tests. As they generally display lower real-life performances than expected, their correct positioning as frontline screening is still controversial. Despite the lack of data from daily clinical use, third generation microfluidic assays (such as the LumiraDx SARS-CoV-2 Ag test) have recently been suggested to have similar performances to RT-qPCR and have been proposed as alternative diagnostic tools. By analyzing 960 nasopharyngeal swabs from 960 subjects at the emergency department admissions of a tertiary COVID-19 hospital, LumiraDx assay demonstrated a specificity of 97% (95% CI: 96-98), and a sensitivity of 85% (95% CI: 82-89) in comparison with RT-qPCR, which increases to 91% (95% CI: 86-95) for samples with a cycle threshold |Aged[MESH] |Antigens, Viral/analysis[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/genetics/immunology[MESH] |Emergency Service, Hospital[MESH] |Female[MESH] |Humans[MESH] |Italy/epidemiology[MESH] |Male[MESH] |Microfluidic Analytical Techniques/methods[MESH] |Middle Aged[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/*genetics/immunology/pathogenicity[MESH]Frontline Screening for SARS-CoV-2 Infection at Emergency Department A(epmc).pdf DeepDyve Pubget Overpricing