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10.1002/eji.202049103 http://scihub22266oqcxt.onion/10.1002/eji.202049103 34081326!�!34081326 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34081326&cmd=llinks): Failed to open stream: HTTP request failed! 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Flow cytometry data mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells |pdf=|usr=}}{{34081326}} gab.com Text Flow cytometry data mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells JO: Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34081326 #FOAvip The phenotype of infused cells is a major determinant of Adoptive T-cell therapy (ACT) efficacy. Yet, the difficulty in deciphering multiparametric cytometry data limited the fine characterization of cellular products. To allow the analysis of dynamic and complex flow cytometry samples, we developed cytoChain, a novel dataset mining tool and a new analytical workflow. CytoChain was challenged to compare state-of-the-art and innovative culture conditions to generate stem-like memory cells (T(SCM) ) suitable for ACT. Noticeably, the combination of IL-7/15 and superoxides scavenging sustained the emergence of a previously unidentified nonexhausted Fit-T(SCM) signature, overlooked by manual gating and endowed with superior expansion potential. CytoChain proficiently traced back this population in independent datasets, and in T-cell receptor engineered lymphocytes. CytoChain flexibility and function were then further validated on a published dataset from circulating T cells in COVID-19 patients. Collectively, our results support the use of cytoChain to identify novel, functionally critical immunophenotypes for ACT and patients immunomonitoring. Twit Text FOAVip Flow cytometry data mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells ????Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34081326 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34081326 #FOAvip English Wikipedia <ref>{{Cite pmid|34081326}}</ref> Flow cytometry data mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells #MMPMID34081326 Manfredi F; Abbati D; Cianciotti BC; Stasi L; Potenza A; Ruggiero E; Magnani Z; Carnevale E; Doglio M; Noviello M; Tassi E; Balestrieri C; Buonanno S; Clemente F; De Lalla C; Protti MP; Mondino A; Casorati G; Dellabona P; Bonini C Eur J Immunol 2021[Aug]; 51 (8): 1992-2005 PMID34081326show ga The phenotype of infused cells is a major determinant of Adoptive T-cell therapy (ACT) efficacy. Yet, the difficulty in deciphering multiparametric cytometry data limited the fine characterization of cellular products. To allow the analysis of dynamic and complex flow cytometry samples, we developed cytoChain, a novel dataset mining tool and a new analytical workflow. CytoChain was challenged to compare state-of-the-art and innovative culture conditions to generate stem-like memory cells (T(SCM) ) suitable for ACT. Noticeably, the combination of IL-7/15 and superoxides scavenging sustained the emergence of a previously unidentified nonexhausted Fit-T(SCM) signature, overlooked by manual gating and endowed with superior expansion potential. CytoChain proficiently traced back this population in independent datasets, and in T-cell receptor engineered lymphocytes. CytoChain flexibility and function were then further validated on a published dataset from circulating T cells in COVID-19 patients. Collectively, our results support the use of cytoChain to identify novel, functionally critical immunophenotypes for ACT and patients immunomonitoring. |COVID-19/blood/immunology[MESH] |Cytokines/metabolism[MESH] |Data Mining/*methods[MESH] |Flow Cytometry/*methods[MESH] |Genetic Engineering[MESH] |Humans[MESH] |Immunologic Memory[MESH] |Immunophenotyping[MESH] |Immunotherapy, Adoptive[MESH] |Receptors, Antigen, T-Cell/genetics/*immunology/metabolism[MESH] |Receptors, Chimeric Antigen/genetics/*immunology[MESH] |SARS-CoV-2/immunology[MESH]Flow cytometry data mining by cytoChain identifies determinants of exh(epmc).pdf DeepDyve Pubget Overpricing