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10.1177/15353702211024597 http://scihub22266oqcxt.onion/10.1177/15353702211024597 34219476!8461048!34219476     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34219476&cmd=llinks): Failed to open stream: HTTP request failed! 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COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting |pdf=|usr=}}{{34219476}} gab.com Text COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting JO: Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=34219476 #FOAvip As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the healthcare environment. Currently, the virus is believed to be transmitted primarily through respiratory droplets, but a growing body of evidence suggests that spread is also possible through aerosolized particles and fomites. Amidst a growing volume of patients with coronavirus disease 2019 (COVID-19), the purpose of this study was to evaluate the potential for SARS-CoV-2 transmission through fomites. Samples collected from the exposed skin of clinicians (n = 42) and high-touch surfaces (n = 40) were collected before and after encounters with COVID-19 patients. Samples were analyzed using two assays: the CDC 2019-nCoV Real-Time Reverse Transcription polymerase chain reaction (RT-qPCR) assay, and a SYBR Green assay that targeted a 121 bp region within the S-gene of SARS-CoV-2. None of the samples tested positive with the CDC assay, while two high-touch surface areas tested positive for SARS-CoV-2 using the Spike assay. However, viral culture did not reveal viable SARS-CoV-2 from the positive samples. Overall, the results from this study suggest that SARS-CoV-2 RNA were not widely present either on exposed skin flora or high-touch surface areas in the hospital locations tested. The inability to recover viable virus from samples that tested positive by the molecular assays, however, does not rule out the possibility of SARS-CoV-2 transmission through fomites. Twit Text FOAVip COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting ????Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=34219476 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34219476 #FOAvip English Wikipedia <ref>{{Cite pmid|34219476}}</ref> COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting #MMPMID34219476 Bartlett C; Langsjoen J; Cheng Q; Yingling AV; Weiss M; Bradfute S; Perkins DJ; Hurwitz I Exp Biol Med (Maywood) 2021[Sep]; 246 (18): 2039-2045 PMID34219476show ga As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the healthcare environment. Currently, the virus is believed to be transmitted primarily through respiratory droplets, but a growing body of evidence suggests that spread is also possible through aerosolized particles and fomites. Amidst a growing volume of patients with coronavirus disease 2019 (COVID-19), the purpose of this study was to evaluate the potential for SARS-CoV-2 transmission through fomites. Samples collected from the exposed skin of clinicians (n = 42) and high-touch surfaces (n = 40) were collected before and after encounters with COVID-19 patients. Samples were analyzed using two assays: the CDC 2019-nCoV Real-Time Reverse Transcription polymerase chain reaction (RT-qPCR) assay, and a SYBR Green assay that targeted a 121 bp region within the S-gene of SARS-CoV-2. None of the samples tested positive with the CDC assay, while two high-touch surface areas tested positive for SARS-CoV-2 using the Spike assay. However, viral culture did not reveal viable SARS-CoV-2 from the positive samples. Overall, the results from this study suggest that SARS-CoV-2 RNA were not widely present either on exposed skin flora or high-touch surface areas in the hospital locations tested. The inability to recover viable virus from samples that tested positive by the molecular assays, however, does not rule out the possibility of SARS-CoV-2 transmission through fomites. |*Health Planning[MESH] |*Hospitals, University[MESH] |*Pandemics[MESH] |COVID-19/*epidemiology/virology[MESH] |Fomites/*virology[MESH] |Health Personnel[MESH] |Humans[MESH] |RNA, Viral/analysis/genetics[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/physiology[MESH] |Specimen Handling[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a(epmc).pdf DeepDyve Pubget Overpricing